Biotrophy-specific downregulation of siderophore biosynthesis in Colletotrichum graminicola is required for modulation of immune responses of maize.
Biotrophy-specific downregulation of siderophore biosynthesis in Colletotrichum graminicola is required for modulation of immune responses of maize.
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DOI：
10.1111/mmi.12561
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发表时间：
2014-04
影响因子：
3.6
通讯作者：
Deising HB
中科院分区：
文献类型：
--
作者：
Albarouki E;Schafferer L;Ye F;von Wirén N;Haas H;Deising HB
半生营养型玉米病原菌禾生炭疽菌（Colletotrichum graminicola）合成一种细胞内铁载体和三种分泌铁载体。eGFP融合关键铁载体生物合成基因，SID 1，编码L-鸟氨酸-N5-单加氧酶，表明铁载体生物合成严格下调，特别是在活体营养发展。为了研究铁载体在营养发育和发病机制中的作用，删除了合成所有铁载体所需的SID 1和合成分泌铁载体的非核糖体肽合成酶基因NPS 6。突变体分析表明，铁载体所需的营养生长在铁限制条件下，分生孢子，活性氧耐受性，和细胞壁的完整性。Δ NPS 1和Δ NPS 6突变体的黑变附着胞形成受阻，毒力下降。与铁载体生物营养型生物合成的特异性下调一致，Δ nps 1和Δ nps 6菌株在生物营养型发育中不受影响，但坏死营养型菌丝的传播减少。为了解决为什么铁载体生物合成在活体营养菌丝中特异性下调的问题，用铁载体浸润玉米叶。铁载体渗透单独不诱导防御反应，但铁载体渗透的叶片中的活体营养菌丝的形成导致ROS的形成和编码防御相关的过氧化物酶和PR蛋白的基因的转录激活显着增加。这些数据表明，真菌铁载体调节植物免疫系统。
The hemibiotrophic maize pathogen C olletotrichum graminicola synthesizes one intracellular and three secreted siderophores. eGFP fusions with the key siderophore biosynthesis gene, SID1, encoding l-ornithine-N 5-monooxygenase, suggested that siderophore biosynthesis is rigorously downregulated specifically during biotrophic development. In order to investigate the role of siderophores during vegetative development and pathogenesis, SID1, which is required for synthesis of all siderophores, and the non-ribosomal peptide synthetase gene NPS6, synthesizing secreted siderophores, were deleted. Mutant analyses revealed that siderophores are required for vegetative growth under iron-limiting conditions, conidiation, ROS tolerance, and cell wall integrity. Δsid1 and Δnps6 mutants were hampered in formation of melanized appressoria and impaired in virulence. In agreement with biotrophy-specific downregulation of siderophore biosynthesis, Δsid1 and Δnps6 strains were not affected in biotrophic development, but spread of necrotrophic hyphae was reduced. To address the question why siderophore biosynthesis is specifically downregulated in biotrophic hyphae, maize leaves were infiltrated with siderophores. Siderophore infiltration alone did not induce defence responses, but formation of biotrophic hyphae in siderophore-infiltrated leaves caused dramatically increased ROS formation and transcriptional activation of genes encoding defence-related peroxidases and PR proteins. These data suggest that fungal siderophores modulate the plant immune system.
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影响因子：
7.2
作者：
Dellagi, A;Rigault, M;Expert, D
通讯作者：
Expert, D
影响因子：
14.8
作者：
Ferreras, JA;Ryu, JS;Quadri, LEN
通讯作者：
Quadri, LEN
影响因子：
3.1
作者：
Hissen, AHT;Wan, ANC;Moore, MM
通讯作者：
Moore, MM
DOI：
10.1007/1-4020-4743-6_10
发表时间：
2006-01-01
期刊：
IRON NUTRITION IN PLANTS AND RHIZOSPHERIC MICROORGANISMS
影响因子：
--
作者：
Expert, Dominique
通讯作者：
Expert, Dominique
影响因子：
11.6
作者：
Eichhorn, Heiko;Lessing, Franziska;Kahmann, Regine
通讯作者：
Kahmann, Regine