The BORDER family of negative transcription elongation factors regulates flowering time in Arabidopsis.
The BORDER family of negative transcription elongation factors regulates flowering time in Arabidopsis.
复制标题
DOI：
10.1016/j.cub.2021.09.074
复制
发表时间：
2021-12-06
期刊：
影响因子：
--
通讯作者：
Michaels SD
中科院分区：
文献类型：
--
作者：
Yu X;Martin PGP;Zhang Y;Trinidad JC;Xu F;Huang J;Thum KE;Li K;Zhao S;Gu Y;Wang X;Michaels SD
长期以来，转录启动一直被认为是基因表达的主要调控步骤。然而，最近的研究表明，下游事件，如转录延伸也可以发挥重要作用。一个来自动物的很好的例子是启动子近端停顿，在那里转录参与的POLII在转录起始点(TSS)下游积累30-50bp，被认为能够快速激活基因。植物并不广泛使用启动子近端停顿，然而，在一种被称为3‘停顿的现象中，在许多基因的转录末端(TES)附近观察到Pol II的显著增加。以前的工作表明，3‘停顿是由边界(BDR)家族的负转录延伸因子促进的。在这里，我们展示了BDR蛋白在基因抑制中的关键作用。与BDR蛋白延缓或暂停延长POL II的作用一致，BDR抑制的基因的特征是高水平的POL II占有率，但低水平的mRNA。BDR蛋白与FPA在物理上相互作用，FPA是大约20多个基因之一，统称为自主成花促进途径，是抑制开花时间基因FLC所必需的。在早花品系中，FLC的表达受到组蛋白抑制修饰的抑制，例如组蛋白H3赖氨酸27三甲基化(H3K27me3)，从而允许植物早花。这些结果表明，BDR蛋白对转录延伸的抑制可能允许转录暂时暂停或通过抑制性组蛋白修饰促进基因的长期抑制。Yu等人。结果表明，被负转录延伸因子BDR家族抑制的基因具有高水平的POL II占有率，尽管稳定状态RNA水平相对较低。通过这种方式，BDR蛋白可能允许稍后恢复转录，或通过抑制性组蛋白修饰促进基因的长期抑制。
Transcription initiation has long been considered a primary regulatory step in gene expression. Recent work, however, shows that downstream events, such as transcription elongation can also play important roles. A well-characterized example from animals is promoter-proximal pausing, where transcriptionally engaged Pol II accumulates 30-50bp downstream of the transcription start site (TSS) and is thought to enable rapid gene activation . Plants do not make widespread use of promoter-proximal pausing, however, in a phenomenon known as 3′ pausing, a significant increase in Pol II is observed near the transcript end site (TES) of many genes. Previous work has shown that 3′ pausing is promoted by the BORDER (BDR) family of negative transcription elongation factors. Here we show that BDR proteins play key roles in gene repression. Consistent with BDR proteins acting to slow or pause elongating Pol II, BDR-repressed genes are characterized by high levels of Pol II occupancy, yet low levels of mRNA. The BDR proteins physically interact with FPA , one of approximately two dozen genes collectively referred to as the autonomous floral-promotion pathway , which are necessary for the repression of the flowering time gene FLOWERING LOCUS C (FLC) . In early-flowering strains, FLC expression is repressed by repressive histone modifications, such as histone H3 lysine 27 trimethylation (H3K27me3), thereby allowing the plants to flower early. These results suggest that the repression of transcription elongation by BDR proteins may allow for the temporary pausing of transcription or facilitate the long-term repression of genes by repressive histone modifications. Yu et al. show that genes repressed by the BDR family of negative transcription elongation factors have high levels of Pol II occupancy, despite relatively low steady state RNA levels. In this way, BDR proteins may allow for the later resumption of transcription or facilitate the longterm repression of genes by repressive histone modifications.
登录
查看更多内容
影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
64.5
作者：
Levine M
通讯作者：
Levine M
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1016/j.bbagrm.2012.02.015
发表时间：
2012-07
影响因子：
4.7
作者：
Gilchrist, Daniel A.;Adelman, Karen
通讯作者：
Adelman, Karen