Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
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全基因组关联研究确定了与玉米内核中与α-生育酚含量相关的三种独立多态性。
DOI：
10.1371/journal.pone.0036807
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yan J
中科院分区：
文献类型：
--
作者：
Li Q;Yang X;Xu S;Cai Y;Zhang D;Han Y;Li L;Zhang Z;Gao S;Li J;Yan J
生育酚是一类四种天然化合物，可以在植物和动物中提供营养和抗氧化剂。玉米粒的α-生育酚含量较低，而α-生育酚是维生素E活性最高的化合物，因此，增加了依赖玉米作为主要维生素E来源的人群缺乏维生素E的风险。本研究通过对一组近500个玉米自交系进行相关性研究，发现玉米γ-生育酚甲基转移酶基因（ZmVTE 4）中的两个插入/缺失（InDels）和位于ZmVTE 4上游约85 kb处的一个单核苷酸多态性（SNP）与玉米籽粒中α-生育酚水平显著相关。对3个群体的连锁分析表明，这3个多态性对α-生育酚含量的影响是独立的。此外，我们发现两个InDel的单倍型可以解释关联面板中约33%的α-生育酚变异，表明ZmVTE 4是参与α-生育酚自然表型变异的主基因。两个InDel中的一个位于启动子区域内并与ZmVTE 4转录水平相关。这些信息不仅有助于理解玉米籽粒中天然生育酚变异的内在机制，而且为玉米籽粒中α-生育酚含量的分子标记辅助育种提供了有价值的标记，从而促进玉米作为一种更好的日常维生素E营养来源的改良。
Tocopherols are a class of four natural compounds that can provide nutrition and function as antioxidant in both plants and animals. Maize kernels have low α-tocopherol content, the compound with the highest vitamin E activity, thus, raising the risk of vitamin E deficiency in human populations relying on maize as their primary vitamin E source. In this study, two insertion/deletions (InDels) within a gene encoding γ-tocopherol methyltransferase, Zea mays VTE4 (ZmVTE4), and a single nucleotide polymorphism (SNP) located ∼85 kb upstream of ZmVTE4 were identified to be significantly associated with α-tocopherol levels in maize kernels by conducting an association study with a panel of ∼500 diverse inbred lines. Linkage analysis in three populations that segregated at either one of these three polymorphisms but not at the other two suggested that the three polymorphisms could affect α-tocopherol content independently. Furthermore, we found that haplotypes of the two InDels could explain ∼33% of α-tocopherol variation in the association panel, suggesting ZmVTE4 is a major gene involved in natural phenotypic variation of α-tocopherol. One of the two InDels is located within the promoter region and associates with ZmVTE4 transcript level. This information can not only help in understanding the underlying mechanism of natural tocopherol variations in maize kernels, but also provide valuable markers for marker-assisted breeding of α-tocopherol content in maize kernels, which will then facilitate the improvement of maize as a better source of daily vitamin E nutrition.
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影响因子：
120.7
作者：
Meydani, SN;Meydani, M;Stollar, BD
通讯作者：
Stollar, BD
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
6.1
作者：
Goffman, FD;Böhme, T
通讯作者：
Böhme, T
DOI：
10.1073/pnas.0606221103
发表时间：
2006-12-05
影响因子：
11.1
作者：
Gilliland, Laura U.;Magallanes-Lundback, Maria;DellaPenna, Dean
通讯作者：
DellaPenna, Dean
影响因子：
56.9
作者：
Buckler, Edward S.;Holland, James B.;McMullen, Michael D.
通讯作者：
McMullen, Michael D.