The Clavibacter michiganensis subsp. michiganensis-tomato interactome reveals the perception of pathogen by the host and suggests mechanisms of infection.
The Clavibacter michiganensis subsp. michiganensis-tomato interactome reveals the perception of pathogen by the host and suggests mechanisms of infection.
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密歇根棒状杆菌密歇根亚种-番茄相互作用组揭示了宿主对病原体的感知并提示了感染机制
DOI：
10.1021/pr200646a
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发表时间：
2012
影响因子：
4.4
通讯作者：
Hettich
中科院分区：
文献类型：
--
作者：
Savidor;Gartemann;Eichenlaub;Chalupowicz;Manulis-Sasson S;Barash;Giannone;Hettich
革兰氏阳性菌密歇根棒状杆菌密歇根亚种（Cmm）引起番茄（Solanum lycopersicum）枯萎病和溃疡病。 Cmm 的致病机制和番茄对 Cmm 感染的反应尚不清楚。为了探索命令与番茄之间的相互作用，使用多维蛋白质鉴定技术（MudPIT）和串联质谱分析体外和植物中产生的样品。结果表明，在感染过程中，Cmm感知植物环境，传递信号，诱导并分泌多种水解酶，包括Pat-1、Ppa和Sbt家族的丝氨酸蛋白酶，CelA、XysA和NagA糖基水解酶以及其他细胞壁降解酶。番茄在感染过程中诱导发病相关 (PR) 蛋白、LOX1 和其他防御相关蛋白，表明植物感知到入侵的细菌并启动基础防御反应，尽管部分防御反应包括一些受抑制的成分，包括 III 类过氧化物酶和分泌的丝氨酸肽酶。番茄乙烯合成酶 ACC 氧化酶在野生型 Cmm 感染期间被诱导，但在内生 Cmm 菌株感染期间则不然，这表明 Cmm 触发的宿主乙烯合成是疾病症状发展的重要因素。蛋白质组数据还用于改进Cmm基因组注释，并确认或扩展了数千个Cmm基因模型。
The Gram-positive bacteriumClavibacter michiganensissubsp.michiganensis(Cmm) causes wilt and canker disease of tomato (Solanum lycopersicum). Mechanisms ofCmmpathogenicity and tomato response toCmminfection are not well understood. To explore the interaction betweenCmmand tomato, multidimensional protein identification technology (MudPIT) and tandem mass spectrometry were used to analyze in vitro and in planta generated samples. The results show that during infectionCmmsenses the plant environment, transmits signals, induces, and then secretes multiple hydrolytic enzymes, including serine proteases of the Pat-1, Ppa, and Sbt familes, the CelA, XysA, and NagA glycosyl hydrolases, and other cell wall-degrading enzymes. Tomato induction of pathogenesis-related (PR) proteins, LOX1, and other defense-related proteins during infection indicates that the plant senses the invading bacterium and mounts a basal defense response, although partial with some suppressed components including class III peroxidases and a secreted serine peptidase. The tomato ethylene-synthesizing enzyme ACC-oxidase was induced during infection with the wild-typeCmmbut not during infection with an endophyticCmmstrain, identifyingCmm-triggered host synthesis of ethylene as an important factor in disease symptom development. The proteomic data were also used to improveCmmgenome annotation, and thousands ofCmmgene models were confirmed or expanded.
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DOI：
--
发表时间：
2008
期刊：
Plant Signalling & Behavior
影响因子：
--
作者：
V. Balaji;G. Sessa
通讯作者：
G. Sessa
影响因子：
9.5
作者：
Elena Bray Speth;Y. Lee;S. He
通讯作者：
Elena Bray Speth;Y. Lee;S. He
影响因子：
1.8
作者：
McDonald, WH;Ohi, R;Yates, JR
通讯作者：
Yates, JR
DOI：
10.1093/bfgp/ell019
发表时间：
2006-12-01
期刊：
Briefings in Functional Genomics & Proteomics
影响因子：
--
作者：
Waters, Katrina M.;Pounds, Joel G.;Thrall, Brian D.
通讯作者：
Thrall, Brian D.
薄荷叶蛋白质组谱响应链格孢感染的变化。
影响因子：
3.3
作者：
R. Sinha;S. Chattopadhyay
通讯作者：
S. Chattopadhyay