Genetic diversity of bacterial communities and gene transfer agents in northern South China Sea.
Genetic diversity of bacterial communities and gene transfer agents in northern South China Sea.
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南海北部细菌群落和基因转移因子的遗传多样性
DOI：
10.1371/journal.pone.0111892
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cheng H
中科院分区：
文献类型：
--
作者：
Sun FL;Wang YS;Wu ML;Jiang ZY;Sun CC;Cheng H
对南海北部16S核糖体RNA基因（rDNA）扩增子进行焦磷酸测序，研究南海北部细菌群落的独特分布，评价细菌群落结构和多样性的空间差异。蓝藻门、变形菌门、放线菌门和拟杆菌门构成了大多数细菌。细菌群落的分类描述表明，nSCS水体中存在较多的藻球菌属、SAR11枝、酸性微生物属、红杆菌属和黄杆菌属。红杆菌属在热带水域的丰度低于温带和冷水水域。此外，还研究了基于基因转移剂（GTA）主衣壳基因（g5）的红杆菌的多样性。从代表不同区域的样本中构建了4个g5基因克隆文库，得到了不同的序列。197个nSCS克隆中鉴定出14个g5个聚类。这些簇也与来自红杆菌属基因组测序的已知g5序列相关。地表水g5序列组成随采样点不同而不同；这表明在nSCS中红杆菌群可能具有高度的多样性。系统发育树分析结果显示，红杆菌属在热带、温带和冷水中具有明显的多样性模式，从而支持特定红杆菌属成员在独特环境中的生态位适应。
Pyrosequencing of the 16S ribosomal RNA gene (rDNA) amplicons was performed to investigate the unique distribution of bacterial communities in northern South China Sea (nSCS) and evaluate community structure and spatial differences of bacterial diversity. Cyanobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes constitute the majority of bacteria. The taxonomic description of bacterial communities revealed that more Chroococcales, SAR11 clade, Acidimicrobiales, Rhodobacterales, and Flavobacteriales are present in the nSCS waters than other bacterial groups. Rhodobacterales were less abundant in tropical water (nSCS) than in temperate and cold waters. Furthermore, the diversity of Rhodobacterales based on the gene transfer agent (GTA) major capsid gene (g5) was investigated. Four g5 gene clone libraries were constructed from samples representing different regions and yielded diverse sequences. Fourteen g5 clusters could be identified among 197 nSCS clones. These clusters were also related to known g5 sequences derived from genome-sequenced Rhodobacterales. The composition of g5 sequences in surface water varied with the g5 sequences in the sampling sites; this result indicated that the Rhodobacterales population could be highly diverse in nSCS. Phylogenetic tree analysis result indicated distinguishable diversity patterns among tropical (nSCS), temperate, and cold waters, thereby supporting the niche adaptation of specific Rhodobacterales members in unique environments.
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影响因子：
56.9
作者：
McDaniel, Lauren D.;Young, Elizabeth;Paul, John H.
通讯作者：
Paul, John H.
DOI：
10.1073/pnas.97.2.859
发表时间：
2000-01-18
影响因子：
11.1
作者：
Lang, AS;Beatty, JT
通讯作者：
Beatty, JT
影响因子：
4.4
作者：
Hagström, Å;Pommier, T;Zweifel, UL
通讯作者：
Zweifel, UL
影响因子：
4.2
作者：
Henriques, IS;Almeida, A;Correia, A
通讯作者：
Correia, A
影响因子：
4.4
作者：
Biers, Erin J.;Sun, Shulei;Howard, Erinn C.
通讯作者：
Howard, Erinn C.