Cytogenetic and Molecular Marker Analyses of a Novel Wheat-Psathyrostachys huashanica 7Ns Disomic Addition Line with Powdery Mildew Resistance.
Cytogenetic and Molecular Marker Analyses of a Novel Wheat-Psathyrostachys huashanica 7Ns Disomic Addition Line with Powdery Mildew Resistance.
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DOI：
10.3390/ijms231810285
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发表时间：
2022-09-07
影响因子：
5.6
通讯作者：
Kang, Houyang
中科院分区：
文献类型：
--
作者：
Tan, Binwen;Wang, Miaomiao;Cai, Li;Li, Sanyue;Zhu, Wei;Xu, Lili;Wang, Yi;Zeng, Jian;Fan, Xing;Sha, Lina;Wu, Dandan;Cheng, Yiran;Zhang, Haiqin;Chen, Guoyue;Zhou, Yonghong;Kang, Houyang
小麦白粉病菌是由小麦白粉病菌Blumeria graminis f.小麦锈病是一种毁灭性的病害，在世界范围内降低小麦产量和质量。发掘和利用野生小麦近缘种的新抗病基因是防治小麦赤霉病最有效的策略。华山新麦草不包括中国。Kuo（2n = 2x = 14，NsNs）是一个重要的第三代基因供体，具有多个对小麦遗传改良特别是抗病性有价值的性状。本研究利用华山松与普通小麦品种中国春和CSph 2b杂交，选育并鉴定了一个新的小麦二体附加系18-1-5-。序列基因组和荧光原位杂交分析表明，18-1-5含有21对小麦染色体和一对外源N染色体。非变性荧光原位杂交和分子标记分析进一步证明了外源染色体来源于华山松的7 Ns染色体。白粉病抗性鉴定结果表明，18-1-5品系对我国流行的白粉病病原菌表现高抗。农艺性状评价结果表明，18-1-5与亲本相比，株高显著降低，粒长显著增加。利用测序基因分型技术，开发了118个华山松7 Ns特异性PCR标记，发现其中26个标记可用于华山松与其他小麦近缘种的基因组区分。因此，18-1-5可以作为小麦抗病育种的一个很有前途的桥梁亲本。这些标记有助于在分子标记辅助育种中快速、准确地检测华山松染色体和携带抗Pm基因的染色体片段，并有助于研究不同Ns基因组与其他近缘基因组之间的遗传差异和系统发育关系。
Powdery mildew caused by Blumeria graminis f. sp. tritici is a devastating disease that reduces wheat yield and quality worldwide. The exploration and utilization of new resistance genes from wild wheat relatives is the most effective strategy against this disease. Psathyrostachys huashanica Keng f. ex P. C. Kuo (2n = 2x = 14, NsNs) is an important tertiary gene donor with multiple valuable traits for wheat genetic improvement, especially disease resistance. In this study, we developed and identified a new wheat—P. huashanica disomic addition line, 18-1-5—derived from a cross between P. huashanica and common wheat lines Chinese Spring and CSph2b. Sequential genomic and multicolor fluorescence in situ hybridization analyses revealed that 18-1-5 harbored 21 pairs of wheat chromosomes plus a pair of alien Ns chromosomes. Non-denaturing fluorescence in situ hybridization and molecular marker analyses further demonstrated that the alien chromosomes were derived from chromosome 7Ns of P. huashanica. The assessment of powdery mildew response revealed that line 18-1-5 was highly resistant at the adult stage to powdery mildew pathogens prevalent in China. The evaluation of agronomic traits indicated that 18-1-5 had a significantly reduced plant height and an increased kernel length compared with its wheat parents. Using genotyping-by-sequencing technology, we developed 118 PCR-based markers specifically for chromosome 7Ns of P. huashanica and found that 26 of these markers could be used to distinguish the genomes of P. huashanica and other wheat-related species. Line 18-1-5 can therefore serve as a promising bridging parent for wheat disease resistance breeding. These markers should be conducive for the rapid, precise detection of P. huashanica chromosomes and chromosomal segments carrying Pm resistance gene(s) during marker-assisted breeding and for the investigation of genetic differences and phylogenetic relationships among diverse Ns genomes and other closely related ones.
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