Formation of complexes at plasmodesmata for potyvirus intercellular movement is mediated by the viral protein P3N-PIPO.
Formation of complexes at plasmodesmata for potyvirus intercellular movement is mediated by the viral protein P3N-PIPO.
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DOI：
10.1371/journal.ppat.1000962
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发表时间：
2010-06-24
期刊：
影响因子：
6.7
通讯作者：
Wang A
中科院分区：
文献类型：
--
作者：
Wei T;Zhang C;Hong J;Xiong R;Kasschau KD;Zhou X;Carrington JC;Wang A
病毒通过细胞质连接进行细胞间运输，称为胞间连丝（PD），对于病毒对植物的全身感染至关重要。先前的遗传和超微结构数据表明，马铃薯Y病毒属环状包涵体（CI）蛋白直接参与细胞间运动，可能是通过形成锚定于PD并通过PD延伸的圆锥形结构来实现的。在这项研究中，我们证明 CI 在本塞姆氏烟草叶细胞中的胞间连丝定位是由最近发现的马铃薯病毒蛋白 P3N-PIPO 以 CI:P3N-PIPO 比率依赖性方式调节的。我们发现 P3N-PIPO 是一种位于 PD 的蛋白质，可与植物中的 CI 发生物理相互作用。将 P3N-PIPO 和 CI 递送至 PD 需要早期分泌途径，而不是肌动球蛋白运动系统。此外，破坏病毒细胞间运动的 CI 突变会损害 PD 定位能力。这些数据表明 CI 和 P3N-PIPO 复合物协调 PD 相关结构的形成，从而促进受感染植物中马铃薯病毒组的细胞间运动。植物病毒病原体每年导致全球农作物产量损失估计达 600 亿美元。马铃薯病毒组占已知植物病毒的 30%，包括许多农业上重要的病毒。尽管马铃薯Y病毒很重要，但其细胞间传播仍知之甚少。先前的研究表明，在感染的早期时间点，病毒编码的 CI 蛋白（11 种已知的马铃薯病毒蛋白之一）与胞间连丝 (PD) 的锥形结构相关，并参与病毒细胞间的运动。在本文中，我们证明了一种新鉴定的马铃薯病毒蛋白 P3N-PIPO 是一种 PD 定位蛋白，它将 CI 蛋白引导至 PD，通过与 CI 蛋白相互作用促进 CI 的锥形结构在 PD 上沉积。我们证明 CI 突变体会损害马铃薯病毒的细胞间运动，并失去在 PD 时积累的能力。我们进一步揭示，P3N-PIPO 利用分泌途径而不是肌动球蛋白运动系统来转运至 PD。总而言之，本研究中提供的数据表明 CI 和 P3N-PIPO 协调 PD 处圆锥形结构的形成，以促进马铃薯病毒细胞间传播。
Intercellular transport of viruses through cytoplasmic connections, termed plasmodesmata (PD), is essential for systemic infection in plants by viruses. Previous genetic and ultrastructural data revealed that the potyvirus cyclindrical inclusion (CI) protein is directly involved in cell-to-cell movement, likely through the formation of conical structures anchored to and extended through PD. In this study, we demonstrate that plasmodesmatal localization of CI in N. benthamiana leaf cells is modulated by the recently discovered potyviral protein, P3N-PIPO, in a CI:P3N-PIPO ratio-dependent manner. We show that P3N-PIPO is a PD-located protein that physically interacts with CI in planta. The early secretory pathway, rather than the actomyosin motility system, is required for the delivery of P3N-PIPO and CI to PD. Moreover, CI mutations that disrupt virus cell-to-cell movement compromise PD-localization capacity. These data suggest that the CI and P3N-PIPO complex coordinates the formation of PD-associated structures that facilitate the intercellular movement of potyviruses in infected plants. Plant viral pathogens cause an estimated US$60 billion loss in crop yields worldwide each year. Potyviruses, accounting for ∼30% of known plant viruses, include many agriculturally important viruses. Despite their importance, the cell-to-cell spread of potyviruses remains poorly understood. Previous studies have shown that at early time points of infection, the virus-encoded CI protein, one of 11 known potyviral proteins, is associated with cone-shaped structures at plasmodesmata (PD) and is involved in viral cell-to-cell movement. In this paper, we show that a newly identified potyviral protein, P3N-PIPO, is a PD-located protein and directs the CI protein to PD, facilitating the deposition of the cone-shaped structures of CI at PD by interacting with CI protein. We demonstrate that the mutant of CI, which impairs potyviral cell-to-cell movement, loses its ability to accumulate at PD. We further reveal that P3N-PIPO utilizes the secretory pathway rather than the actomyosin motility system for trafficking to PD. Taken together, the data presented in this study suggest that CI and P3N-PIPO coordinates the formation of conical structure at PD for potyviral cell-to-cell spread.
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