In vivo proton MRS to quantify anesthetic effects of pentobarbital on cerebral metabolism and brain activity in rat.
In vivo proton MRS to quantify anesthetic effects of pentobarbital on cerebral metabolism and brain activity in rat.
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DOI：
10.1002/mrm.22146
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发表时间：
2009-12
影响因子：
3.3
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Du, Fei;Zhang, Yi;Iltis, Isabelle;Marjanska, Malgorzata;Zhu, Xiao-Hong;Henry, Pierre-Gilles;Chen, Wei
为了定量研究戊巴比妥麻醉对脑活动、脑代谢物浓度和脑葡萄糖代谢率的影响，测定了不同剂量戊巴比妥的大鼠脑质子磁共振波谱和脑电图。结果表明，戊巴比妥注射液中的溶剂丙二醇引起的共振在脑中可被稳健地检测和定量;大多数脑代谢物的浓度在等电状态下保持恒定（无声脑电图）与轻度异氟烷麻醉条件相比，高剂量戊巴比妥，除了脑葡萄糖水平降低61%，这与脑葡萄糖代谢率降低37%有关，表明在等电状态下维持脑细胞完整性的大量“管家”能量;脑电图和脑代谢活动与戊巴比妥麻醉深度密切相关，它们可以通过丙二醇共振信号间接量化，1.13 ppm。这项研究表明，在体内质子磁共振波谱可以用来测量大脑代谢物浓度和大脑葡萄糖代谢率的变化，在不同的戊巴比妥麻醉状态下，此外，丙二醇信号提供了一个敏感的生物标志物，定量监测这些变化和麻醉深度无创。
To quantitatively investigate the effects of pentobarbital anesthesia on brain activity, brain metabolite concentrations and cerebral metabolic rate of glucose, in vivo proton MR spectra, and electroencephalography were measured in the rat brain with various doses of pentobarbital. The results show that the resonances attributed to propylene glycol, a solvent in pentobarbital injection solution, can be robustly detected and quantified in the brain; the concentration of most brain metabolites remained constant under the isoelectric state (silent electroencephalography) with a high dose of pentobarbital compared to mild isoflurane anesthesia condition, except for a reduction of 61% in the brain glucose level, which was associated with a 37% decrease in cerebral metabolic rate of glucose, suggesting a significant amount of “housekeeping” energy for maintaining brain cellular integrity under the isoelectric state; and electroencephalography and cerebral metabolic activities were tightly coupled to the pentobarbital anesthesia depth and they can be indirectly quantified by the propylene glycol resonance signal at 1.13 ppm. This study indicates that in vivo proton MR spectroscopy can be used to measure changes in cerebral metabolite concentrations and cerebral metabolic rate of glucose under varied pentobarbital anesthesia states; moreover, the propylene glycol signal provides a sensitive biomarker for quantitatively monitoring these changes and anesthesia depth noninvasively.
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DOI：
10.1097/00004647-200003000-00007
发表时间：
2000-03-01
影响因子：
6.3
作者：
Hyder, F;Kennan, RP;Rothman, D
通讯作者：
Rothman, D
影响因子：
3.3
作者：
Iltis, Isabelle;Marjanska, Malgorzata;Henry, Pierre-Gilles
通讯作者：
Henry, Pierre-Gilles
影响因子：
3.3
作者：
GRUETTER, R
通讯作者：
GRUETTER, R
DOI：
10.1073/pnas.90.21.9896
发表时间：
1993-11-01
影响因子：
11.1
作者：
CHEN, W;NOVOTNY, EJ;SHULMAN, RG
通讯作者：
SHULMAN, RG
影响因子：
3.3
作者：
Henry, PG;Marjanska, M;Ugurbil, K
通讯作者：
Ugurbil, K