Honey bee genetics shape the strain-level structure of gut microbiota in social transmission.
Honey bee genetics shape the strain-level structure of gut microbiota in social transmission.
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蜜蜂遗传学在社会传播中塑造肠道微生物群的菌株水平结构
DOI：
10.1186/s40168-021-01174-y
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发表时间：
2021-11-17
期刊：
影响因子：
15.5
通讯作者：
Zheng H
中科院分区：
文献类型：
--
作者：
Wu J;Lang H;Mu X;Zhang Z;Su Q;Hu X;Zheng H
背景蜜蜂肠道菌群通过社会交往传播，对宿主健康有益。虽然微生物群落相对稳定，但在蜜蜂中发现了个体差异和高菌株水平的多样性。尽管蜜蜂肠道微生物群结构受到环境因素的影响，但肠道成员的遗传性和宿主遗传学的贡献仍然难以捉摸。考虑到蜜蜂在一个殖民地内是不容易遗传相同的，由于一妻多夫制的女王，我们假设的微生物群结构可以通过主机genetics.ResultsWe塑造使用鸟枪宏基因组学同时配置文件的微生物群和主机基因型的蜜蜂从蜂箱的四个不同的亚种。肠道组成是更遥远的遗传不同的蜜蜂在两个基因型和“序列离散人口”的水平。然后，我们在人工授精产生的杂交蜜蜂群体中进行了连续传代实验，结果表明，在社会传播过程中，不同批次的蜜蜂的微生物组成发生了显著变化。具体而言，不同的菌株，从ESTA型ofScarlassella alviare优先选择遗传多样的主机，和菌株从不同的主机显示出显着的单核苷酸多态性分布在IV型皮利基因座的偏见。全基因组关联分析发现，一簇双歧杆菌菌株的相对丰度与蜜蜂大脑中特异性表达的宿主谷氨酸受体基因相关。最后，单殖民化的双歧杆菌与一个特定的多糖利用位点的影响的选择性剪接的thegluR-B基因，这是与增加的GABA水平在brain.ConclusionsOur结果表明，宿主遗传学的影响蜜蜂肠道组成，并建议肠-脑连接牵连在肠道细菌菌株的偏好。蜜蜂已被广泛用作社会行为，遗传学和肠道微生物组的模式生物。进一步确定宿主遗传功能作为微生物结构的塑造力量将促进我们对宿主-微生物相互作用的理解。视频摘要
BackgroundHoney bee gut microbiota transmitted via social interactions are beneficial to the host health. Although the microbial community is relatively stable, individual variations and high strain-level diversity have been detected across honey bees. Although the bee gut microbiota structure is influenced by environmental factors, the heritability of the gut members and the contribution of the host genetics remains elusive. Considering bees within a colony are not readily genetically identical due to the polyandry of the queen, we hypothesize that the microbiota structure can be shaped by host genetics.ResultsWe used shotgun metagenomics to simultaneously profile the microbiota and host genotypes of bees from hives of four different subspecies. Gut composition is more distant between genetically different bees at both phylotype- and “sequence-discrete population” levels. We then performed a successive passaging experiment within colonies of hybrid bees generated by artificial insemination, which revealed that the microbial composition dramatically shifts across batches of bees during the social transmission. Specifically, different strains from the phylotype ofSnodgrassella alviare preferentially selected by genetically varied hosts, and strains from different hosts show a remarkably biased distribution of single-nucleotide polymorphism in the Type IV pili loci. Genome-wide association analysis identified that the relative abundance of a cluster ofBifidobacteriumstrains is associated with the host glutamate receptor gene specifically expressed in the bee brain. Finally, mono-colonization ofBifidobacteriumwith a specific polysaccharide utilization locus impacts the alternative splicing of thegluR-Bgene, which is associated with an increased GABA level in the brain.ConclusionsOur results indicated that host genetics influence the bee gut composition and suggest a gut-brain connection implicated in the gut bacterial strain preference. Honey bees have been used extensively as a model organism for social behaviors, genetics, and the gut microbiome. Further identification of host genetic function as a shaping force of microbial structure will advance our understanding of the host-microbe interactions.Video abstract
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