Radiation metabolomics and its potential in biodosimetry.
Radiation metabolomics and its potential in biodosimetry.
复制标题
DOI：
10.3109/09553002.2011.556177
复制
发表时间：
2011-08
影响因子：
2.6
通讯作者：
Fornace A Jr
中科院分区：
文献类型：
--
作者：
Coy SL;Cheema AK;Tyburski JB;Laiakis EC;Collins SP;Fornace A Jr
辐射暴露会触发一个复杂的分子和细胞反应网络，影响代谢过程并改变代谢物的水平。这些代谢物有可能成为辐射剂量测定的生物标志物。本综述概述了辐射信号和新陈代谢、发现阶段使用的代谢学方法以及具有评估现场辐射损伤潜力的仪器。快速、高分辨率色谱和质谱学的最新发展以及新的数据分析方法使基于非侵入性获得的生物液的数千种代谢物的定量评估成为可能。这种复杂的分析导致通过有针对性的定量测量对筛选和生物剂量测定有用的代谢物组的发现阶段识别。用于目标分析的仪器可能比用于发现的仪器更简单，因此我们检查了基于离子迁移率的当前技术。最近发表的结果和正在进行的研究检查了辐射暴露引起的许多代谢物水平的复杂变化，并确定了用于辐射生物剂量测定的小分子生物标志物组。基于与质量垂直分离、抑制化学噪声和高灵敏度的结果，差示迁移质谱(DMS-MS)离子迁移率光谱在可展开仪器的发展中显示出极大的潜力。
Radiation exposure triggers a complex network of molecular and cellular responses that impacts metabolic processes and alters the levels of metabolites. Such metabolites have potential as biomarkers for radiation dosimetry. This review provides an overview of radiation signalling and metabolism, of metabolomic approaches used in the discovery phase, and of instrumentation with the potential to assess radiation injury in the field. Recent developments in fast, high-resolution chromatography and mass spectrometry and new data analysis methods allow the quantitative assessment of thousands of metabolites based on biofluids obtained non-invasively. This complex analysis leads to the discovery-phase identification of groups of metabolites useful for screening and biodosimetry by targeted quantitative measurement. Instrumentation for target analysis can be simpler than that used for discovery, so we examine current technologies based on ion mobility. Recent published results and ongoing studies examine the complex changes in the levels of many metabolites caused by radiation exposure, and identify groups of small-molecule biomarkers for radiation biodosimetry. Based on results showing separation orthogonal to mass, chemical noise suppression, and high sensitivity, differential mobility mass spectrometry (DMS-MS) ion mobility spectrometry appears highly promising for the development of deployable instrumentation.
登录
查看更多内容
影响因子：
9.3
作者：
Abbatiello SE;Mani DR;Keshishian H;Carr SA
通讯作者：
Carr SA
影响因子：
19
作者：
Feng Z;Levine AJ
通讯作者：
Levine AJ
影响因子：
5.8
作者：
Eichler, GS;Huang, S;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
120.1
作者：
Goodsaid, Federico M.;Amur, Shashi;Zineh, Issam
通讯作者：
Zineh, Issam
影响因子：
4.4
作者：
Bao, Yuqian;Zhao, Tie;Jia, Wei
通讯作者：
Jia, Wei