Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment.
Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment.
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来自光细菌damselae subsp的新型共轭可转移多种耐药性质粒PAQU1。从海洋水产养殖环境中分离出来的Damselae。
DOI：
10.1264/jsme2.me11338
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发表时间：
2012
影响因子：
2.2
通讯作者：
Masuda M
中科院分区：
文献类型：
--
作者：
Nonaka L;Maruyama F;Miyamoto M;Miyakoshi M;Kurokawa K;Masuda M
耐药细菌的出现是水产养殖业面临的一个严重问题。耐药基因从一个细菌细胞转移到另一个细菌细胞的能力被认为是这些基因在水产养殖环境中广泛传播的原因；然而，人们对海洋细菌中的基因转移机制知之甚少。在本研究中，我们发现了一株具有四环素抗性的淡色光杆菌亚种。从日本沿海水产养殖场的海水中分离出的丹丝体含有一种新的多重耐药质粒。这种命名为pAQU1的质粒可以通过接合的方式转移到大肠杆菌中。核苷酸序列分析表明，该质粒全长204,052个碱基对，含有235个预测编码序列。注释显示，pAQU1没有已知的REPA，提示有一个新的复制子，含有7个耐药基因：blaCARB-9-like、Flor、mph(A)-like、MEF(A)-like、sul2、tet(M)和tet(B)。该质粒有一套完整的基因，编码IV型分泌系统的装置，具有独特的TraA复制。对pAQU1中TRAI编码的松弛酶氨基酸序列进行了系统进化分析，结果表明该基因的接合转移系统属于MOBH12，属于MOBH质粒家族的一个亚群，与Inca/C型质粒关系密切，SXT/R391广泛分布于肠杆菌科和弧菌科。我们的数据表明，接合转移参与了海洋细菌之间的水平基因转移，并为水产养殖环境中细菌间耐药基因的传播提供了有用的分子基础。
The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanisms in marine bacteria. In this study, we show that a tetracycline-resistant strain of Photobacterium damselae subsp. damselae, isolated from seawater at a coastal aquaculture site in Japan, harbors a novel multiple drug resistance plasmid. This plasmid named pAQU1 can be transferred to Escherichia coli by conjugation. Nucleotide sequencing showed that the plasmid was 204,052 base pairs and contained 235 predicted coding sequences. Annotation showed that pAQU1 did not have known repA, suggesting a new replicon, and contained seven drug resistance genes: blaCARB-9-like, floR, mph(A)-like, mef(A)-like, sul2, tet(M) and tet(B). The plasmid has a complete set of genes encoding the apparatus for the type IV secretion system with a unique duplication of traA. Phylogenetic analysis of the deduced amino acid sequence of relaxase encoded by traI in pAQU1 demonstrated that the conjugative transfer system of the plasmid belongs to MOBH12, a sub-group of the MOBH plasmid family, closely related to the IncA/C type of plasmids and SXT/R391 widely distributed among species of Enterobacteriaceae and Vibrionaceae. Our data suggest that conjugative transfer is involved in horizontal gene transfer among marine bacteria and provide useful insights into the molecular basis for the dissemination of drug resistance genes among bacteria in the aquaculture environment.
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影响因子：
3.6
作者：
Griffith, KL;Becker, SM;Wolf, RE
通讯作者：
Wolf, RE
影响因子：
4.9
作者：
JORIS, B;LEDENT, P;FRERE, JM
通讯作者：
FRERE, JM
影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
2.6
作者：
Alcaide, E
通讯作者：
Alcaide, E