Parallel High-Throughput Automated Assays to Measure Cell Growth and Beta-Galactosidase Reporter Gene Expression in the Yeast Saccharomyces cerevisiae.
Parallel High-Throughput Automated Assays to Measure Cell Growth and Beta-Galactosidase Reporter Gene Expression in the Yeast Saccharomyces cerevisiae.
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用于测量酿酒酵母细胞生长和 β-半乳糖苷酶报告基因表达的并行高通量自动测定。
DOI：
10.1002/9780470559277.ch100119
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发表时间：
2011
影响因子：
--
通讯作者：
Pavitt,GrahamD
中科院分区：
文献类型：
--
作者：
Napper,AndrewD;Motlekar,Nuzhat;deAlmeida,RogerioAlves;Pavitt,GrahamD
Parallel high‐throughput automated assays are described for the measurement of cell growth and β‐galactosidase reporter gene expression from a single culture of the yeastS. cerevisiae. The dual assay measures the effect of test compounds on expression of a specific gene of interest linked to the β‐galactosidase reporter gene, and simultaneously tests for compound toxicity and other effects on cell growth. Examples of assay development and validation results are used to illustrate how this protocol may be used to screen two yeast cell lines in parallel. Yeast cells are grown overnight in V‐bottom polypropylene 384‐well plates, after which portions of the cell suspension are transferred to clear and to white flat‐bottom 384‐well plates for measurement of cell growth and reporter gene expression, respectively. Cell growth is determined by measurement of absorbance at 595 nm, and β‐galactosidase expression is quantified by Beta‐Glo, a commercially available luminescent β‐galactosidase substrate.Curr. Protoc. Chem. Biol. 3:1‐14 © 2011 by John Wiley & Sons, Inc.
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DOI：
10.1056/nejm197611252952201
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期刊：
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DOI：
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期刊：
Acta Physiologica Scandinavica
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DOI：
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发表时间：
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