Obesity III: Obesogen assays: Limitations, strengths, and new directions.
Obesity III: Obesogen assays: Limitations, strengths, and new directions.
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DOI：
10.1016/j.bcp.2022.115014
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发表时间：
2022-05
影响因子：
5.8
通讯作者：
Heindel, Jerrold J.
中科院分区：
文献类型：
--
作者：
Kassotis, Christopher D.;Saal, Frederick S. vom;Babin, Patrick J.;Lagadic-Gossmann, Dominique;Le Mentec, Helene;Blumberg, Bruce;Mohajer, Nicole;Legrand, Antoine;Kos, Vesna Munic;Martin-Chouly, Corinne;Podechard, Normand;Langoue, Sophie;Touma, Charbel;Barouki, Robert;Kim, Min Ji;Audouze, Karine;Choudhury, Mahua;Shree, Nitya;Bansal, Amita;Howard, Sarah;Heindel, Jerrold J.
越来越多的证据表明，环境污染物在破坏人类和动物的代谢健康方面发挥着重要作用。尽管越来越需要用于评估成脂和潜在致胖污染物的良好理解的模型，但一直依赖于几十年前的体外模型，这些模型尚未得到细胞系提供者的适当管理。在过去的十年中，可用的体外模型迅速增加，包括人类间充质干细胞和前脂肪细胞模型的商业可用性;这些模型需要更全面的验证，但在改善人类代谢健康方面表现出真实的前景。在开发三维和共培养技术方面也取得了进展，这些技术允许对更生理相关的状态进行询问。虽然存在多种啮齿动物模型用于在生理学相关背景下评价推定的致肥胖和/或致脂肪化学品，但已确定替代模型生物体（如果蝇、C.线虫、斑马鱼和青鳉在代谢健康测试中的作用。这些模型有几个明显的优势，最显着的大小，快速发展，大育雏的大小，并容易在整个生物体的高分辨率脂质积累。预计它们将扩大代谢健康研究的能力，特别是当与本文所述的新兴肥胖评估技术相结合时。
There is increasing evidence of a role for environmental contaminants in disrupting metabolic health in both humans and animals. Despite a growing need for well-understood models for evaluating adipogenic and potential obesogenic contaminants, there has been a reliance on decades-old in vitro models that have not been appropriately managed by cell line providers. There has been a quick rise in available in vitro models in the last ten years, including commercial availability of human mesenchymal stem cell and preadipocyte models; these models require more comprehensive validations but demonstrate real promise in improved translation to human metabolic health. There is also progress in developing three-dimensional and co-culture techniques that allow for the interrogation of a more physiologically relevant state. While diverse rodent models exist for evaluating putative obesogenic and/or adipogenic chemicals in a physiologically relevant context, increasing capabilities have been identified for alternative model organisms such as Drosophila, C. elegans, zebrafish, and medaka in metabolic health testing. These models have several appreciable advantages, most notably the size, rapid development, large brood sizes, and ease of high-resolution lipid accumulation throughout the organisms. They are anticipated to expand the capabilities of metabolic health research, particularly when coupled with emerging obesogen evaluation techniques as described herein.
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DOI：
10.1016/j.reprotox.2013.07.017
发表时间：
2013-12
期刊：
Reproductive toxicology (Elmsford, N.Y.)
影响因子：
--
作者：
Angle BM;Do RP;Ponzi D;Stahlhut RW;Drury BE;Nagel SC;Welshons WV;Besch-Williford CL;Palanza P;Parmigiani S;vom Saal FS;Taylor JA
通讯作者：
Taylor JA
影响因子：
1.2
作者：
Bovier, Tiziana Francesca;Cavaliere, Daniels;Digilio, Filomena Anna
通讯作者：
Digilio, Filomena Anna
影响因子：
3.7
作者：
Ariemma F;D'Esposito V;Liguoro D;Oriente F;Cabaro S;Liotti A;Cimmino I;Longo M;Beguinot F;Formisano P;Valentino R
通讯作者：
Valentino R
影响因子：
5.2
作者：
Bateman ME;Strong AL;McLachlan JA;Burow ME;Bunnell BA
通讯作者：
Bunnell BA
影响因子：
5.5
作者：
Abdelmoez, Ahmed M.;Puig, Laura Sardon;Pillon, Nicolas J.
通讯作者：
Pillon, Nicolas J.