Solitary chemosensory cells producing interleukin-25 and group-2 innate lymphoid cells are enriched in chronic rhinosinusitis with nasal polyps.
Solitary chemosensory cells producing interleukin-25 and group-2 innate lymphoid cells are enriched in chronic rhinosinusitis with nasal polyps.
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DOI：
10.1002/alr.22142
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发表时间：
2018-05-09
影响因子：
6.4
通讯作者：
Cohen NA
中科院分区：
文献类型：
--
作者：
Patel NN;Kohanski MA;Maina IW;Triantafillou V;Workman AD;Tong CCL;Kuan EC;Bosso JV;Adappa ND;Palmer JN;Herbert DR;Cohen NA
慢性鼻窦炎伴鼻息肉（CRSwNP）通常以2型炎症为特征。已经确定，2组先天淋巴样细胞（ILC2s）是免疫细胞的一个亚群，在协调粘膜2型反应中起重要作用。IL-25是一种上皮源性细胞因子，是ILC2s的关键激活因子。最近的证据表明，特殊的味觉上皮细胞，如孤立化学感觉细胞（SCCs），可能是IL-25的产生者。为了阐明CRSwNP中SCCs和ILC2s之间的关系，我们试图量化ILC2s和SCCs，以确定与健康的鼻粘膜相比，这些细胞类型是否在鼻息肉中富集。我们采用多色流式细胞术对7例鼻息肉和非炎症鼻甲粘膜的SCCs和ILC2s进行定量，并研究IL-13和地塞米松对鼻息肉和鼻甲粘膜组织移植的SCCs发生频率的影响。发现SCCs是IL-25的主要来源。鼻息肉的SCCs （33.0% vs. 5.6%, p <0.001）和ILC2s （2.40% vs. 0.19%, p = 0.008）比患者匹配的非息肉状鼻甲高。在培养的息肉外植体中，外源性IL-13增加了上皮细胞SCCs的比例（IL-13组40.2% vs.未处理组28.9%,p = 0.012），添加地塞米松可逆转这一效应（40.2% vs. 8.9%, p<0.0005）。这些数据支持SCC和ILC2的扩增以及鼻息肉中IL-25的增加，并可能代表CRSwNP发病机制的早期事件。IL-13在前馈循环中刺激SCC的增殖，这是一个类固醇敏感的过程。
Chronic rhinosinusitis with nasal polyps (CRSwNP) is commonly characterized by type-2 inflammation. It is established that group-2 innate lymphoid cells (ILC2s) are a subset of immune cells important in orchestrating mucosal type-2 response. IL-25 is an epithelial-derived cytokine that is a critical activator of ILC2s. Recent evidence demonstrates that specialized taster epithelial cells, such as solitary chemosensory cells (SCCs), may be producers of IL-25. To elucidate the relationship between SCCs and ILC2s in CRSwNP, we sought to quantify ILC2s and SCCs to determine if these cell types are enriched in nasal polyps compared to healthy sinonasal mucosa. We quantified SCCs and ILC2s using multicolor flow cytometry in nasal polyps and non-inflamed turbinate mucosa from seven patients and investigated the role of IL-13 and dexamethasone on SCC frequency using tissue explants of nasal polyps and turbinate mucosa. SCCs were found to be the primary source of IL-25. Nasal polyps demonstrated higher populations of SCCs (33.0% vs. 5.6%, p <0.001) and ILC2s (2.40% vs. 0.19%, p = 0.008) compared to patient-matched non-polypoid turbinates. In cultured polyp explants, exogenous IL-13 increased the proportion of epithelial SCCs (40.2% IL-13 condition vs. 28.9% untreated, p = 0.012), and this effect was reversed by addition of dexamethasone (40.2% vs. 8.9%, p<0.0005). These data support SCC and ILC2 expansion as well as increased IL-25 production in nasal polyps and may represent early events in the pathogenesis of CRSwNP. IL-13 stimulates proliferation of SCC in a feed-forward loop, a process that is steroid-sensitive.
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影响因子：
64.8
作者：
Gerbe F;Sidot E;Smyth DJ;Ohmoto M;Matsumoto I;Dardalhon V;Cesses P;Garnier L;Pouzolles M;Brulin B;Bruschi M;Harcus Y;Zimmermann VS;Taylor N;Maizels RM;Jay P
通讯作者：
Jay P
影响因子：
5.9
作者：
Chen, Fenghong;Hong, Haiyu;Shi, Jianbo
通讯作者：
Shi, Jianbo
簇细胞，味觉化学细胞，在肠道中编排寄生虫2型免疫力。
DOI：
10.1126/science.aaf1648
发表时间：
2016-03-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Howitt MR;Lavoie S;Michaud M;Blum AM;Tran SV;Weinstock JV;Gallini CA;Redding K;Margolskee RF;Osborne LC;Artis D;Garrett WS
通讯作者：
Garrett WS
影响因子：
6.4
作者：
Barham, Henry P.;Cooper, Sarah E.;Anderson, Catherine B.;Tizzano, Marco;Kingdom, Todd T.;Finger, Tom E.;Kinnamon, Sue C.;Ramakrishnan, Vijay R.
通讯作者：
Ramakrishnan, Vijay R.
DOI：
10.1016/j.jaci.2015.10.019
发表时间：
2016-05
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Lam EP;Kariyawasam HH;Rana BM;Durham SR;McKenzie AN;Powell N;Orban N;Lennartz-Walker M;Hopkins C;Ying S;Rimmer J;Lund VJ;Cousins DJ;Till SJ
通讯作者：
Till SJ