Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa.
Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa.
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DOI：
10.1002/alr.21149
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发表时间：
2013-06
影响因子：
6.4
通讯作者：
Ramakrishnan, Vijay R.
中科院分区：
文献类型：
--
作者：
Barham, Henry P.;Cooper, Sarah E.;Anderson, Catherine B.;Tizzano, Marco;Kingdom, Todd T.;Finger, Tom E.;Kinnamon, Sue C.;Ramakrishnan, Vijay R.
关键词：
孤立化学感应细胞 (SCC) 是呼吸道上皮中的特殊细胞，可对包括细菌信号分子在内的有毒化学物质做出反应。 SCC 表达苦味转导成分，包括 TAS2R 苦味受体和下游信号传导效应器：α-Gustducin、PLCβ2 和 TRPM5。当被激活时，鳞状细胞会引起神经源性反射，导致局部炎症。本研究的目的是测试人类鼻窦上皮中是否存在鳞状细胞癌，并测试其与过敏性鼻炎和慢性鼻窦炎等炎症性疾病过程的相关性。从对照患者（n=7）和患有过敏性鼻炎和/或慢性鼻窦炎的患者（n=15）获得患者人口统计数据和人类鼻窦粘膜活检。 RT-PCR、qPCR 和免疫组织化学用于确定患病患者中信号传导效应子的表达是否发生改变。 RT-PCR 表明苦味受体 TAS2R4、TAS2R14 和 TAS2R46 以及下游信号效应器 α-Gustducin、PLCβ2 和 TRPM5 在对照和患病患者的下鼻甲、中鼻甲、鼻中隔和钩突中表达。在对照和患病患者的鼻窦粘膜中均发现了 PLCβ2/TRPM5 免疫反应性 SCC。 qPCR显示对照组和患病组钩突中α-Gustducin和TRPM5的表达相似，并且表达水平与SNOT-22或疼痛评分之间没有相关性。 SCC 存在于人类鼻窦粘膜的功能相关区域。对照和患病患者中信号效应器的表达水平相似，并且与疼痛和炎症的测量不相关。对这些途径的进一步研究可能有助于深入了解鼻炎性疾病，并可能提供潜在的治疗靶点。
Solitary chemosensory cells (SCCs) are specialized cells in the respiratory epithelium that respond to noxious chemicals including bacterial signaling molecules. SCCs express components of bitter taste transduction including the TAS2R bitter taste receptors and downstream signaling effectors: α-Gustducin, PLCβ2, and TRPM5. When activated, SCCs evoke neurogenic reflexes, resulting in local inflammation. The purpose of this study was to test for the presence SCCs in human sinonasal epithelium, and to test for a correlation with inflammatory disease processes such as allergic rhinitis and chronic rhinosinusitis. Patient demographics and biopsies of human sinonasal mucosa were obtained from control patients (n=7) and those with allergic rhinitis and/or chronic rhinosinusitis (n=15). RT-PCR, qPCR and immunohistochemistry were used to determine whether expression of signaling effectors was altered in diseased patients. RT-PCR demonstrated that bitter taste receptors TAS2R4, TAS2R14 and TAS2R46 and downstream signaling effectors α-Gustducin, PLCβ2, and TRPM5 are expressed in the inferior turbinate, middle turbinate, septum and uncinate of both control and diseased patients. PLCβ2/TRPM5-immunoreactive SCCs were identified in the sinonasal mucosa of both control and diseased patients. qPCR showed similar expression of α-Gustducin and TRPM5 in the uncinate process of control and diseased groups, and there was no correlation between level of expression and SNOT-22 or pain scores. SCCs are present in human sinonasal mucosa in functionally relevant areas. Expression level of signaling effectors was similar in control and diseased patients and did not correlate with measures of pain and inflammation. Further study into these pathways may provide insight into nasal inflammatory diseases and may offer potential therapeutic targets.
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影响因子：
3.4
作者：
GLIKLICH, RE;METSON, R
通讯作者：
METSON, R
影响因子：
15.9
作者：
Lee, Robert J.;Xiong, Guoxiang;Cohen, Noam A.
通讯作者：
Cohen, Noam A.
DOI：
10.1177/194589240301700106
发表时间：
2003-01-01
期刊：
AMERICAN JOURNAL OF RHINOLOGY
影响因子：
--
作者：
Bhattacharyya, N
通讯作者：
Bhattacharyya, N
影响因子：
14.2
作者：
LEE, BJ;NACLERIO, RM;BAROODY, FM
通讯作者：
BAROODY, FM
DOI：
10.3410/b3-20
发表时间：
2011
期刊：
F1000 biology reports
影响因子：
--
作者：
Finger TE;Kinnamon SC
通讯作者：
Kinnamon SC