The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity.
The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity.
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DOI：
10.1242/dev.104687
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Eshed Williams L
中科院分区：
文献类型：
--
作者：
Mandel T;Moreau F;Kutsher Y;Fletcher JC;Carles CC;Eshed Williams L
在植物中，茎尖分生组织（SAM）充当多能干细胞的储存库，所有地上器官均起源于此。为了维持适当的生长，SAM 必须维持多能干细胞的自我更新和侧向器官形成的细胞募集之间的稳态。拟南芥中调节这种稳态的网络核心是指定干细胞命运的 WUSCHEL (WUS) 转录因子和限制 WUS 表达的 CLAVATA (CLV) 配体受体系统。在这项研究中，我们将 ERECTA (ER) 途径确定为第二个受体激酶信号传导途径，可调节 WUS 表达，从而独立于 CLV 途径调节茎尖和花分生组织的大小。我们证明，III类HD-ZIP和ER功能的减少共同导致WUS表达显着增加，导致芽分生组织极度增大以及从螺旋状营养叶序转变为轮生叶序。我们进一步表明，花序分生组织中 WUS 的强烈上调导致 AGAMOUS 同源异型基因的异位表达达到将细胞命运从花分生组织创始人细胞转变为心皮创始人细胞的水平，表明 ER 在调节花分生组织身份中具有间接作用。这项工作说明了干细胞规范和分生组织分化之间的微妙平衡，并表明这种平衡的变化会导致叶序异常和生殖细胞命运的改变。
In plants, the shoot apical meristem (SAM) serves as a reservoir of pluripotent stem cells from which all above ground organs originate. To sustain proper growth, the SAM must maintain homeostasis between the self-renewal of pluripotent stem cells and cell recruitment for lateral organ formation. At the core of the network that regulates this homeostasis in Arabidopsis are the WUSCHEL (WUS) transcription factor specifying stem cell fate and the CLAVATA (CLV) ligand-receptor system limiting WUS expression. In this study, we identified the ERECTA (ER) pathway as a second receptor kinase signaling pathway that regulates WUS expression, and therefore shoot apical and floral meristem size, independently of the CLV pathway. We demonstrate that reduction in class III HD-ZIP and ER function together leads to a significant increase in WUS expression, resulting in extremely enlarged shoot meristems and a switch from spiral to whorled vegetative phyllotaxy. We further show that strong upregulation of WUS in the inflorescence meristem leads to ectopic expression of the AGAMOUS homeotic gene to a level that switches cell fate from floral meristem founder cell to carpel founder cell, suggesting an indirect role for ER in regulating floral meristem identity. This work illustrates the delicate balance between stem cell specification and differentiation in the meristem and shows that a shift in this balance leads to abnormal phyllotaxy and to altered reproductive cell fate.
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影响因子：
9.2
作者：
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通讯作者：
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