Drosophila glypicans regulate the germline stem cell niche.
Drosophila glypicans regulate the germline stem cell niche.
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DOI：
10.1083/jcb.200904118
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发表时间：
2009-11-16
期刊：
影响因子：
--
通讯作者：
Nakato H
中科院分区：
文献类型：
--
作者：
Hayashi Y;Kobayashi S;Nakato H
硫酸乙酰肝素糖蛋白dally和dally样分别定义雌性和雄性果蝇的生殖细胞龛。干细胞在体内通过短距离信号系统维持在称为小生境的专门微环境中，但控制干细胞小生境物理空间的分子机制知之甚少。在这项研究中，我们报告，硫酸乙酰肝素（HS）蛋白多糖（HSPGs）是必需的调节剂的生殖系干细胞（GSC）的小生境中的果蝇性腺。HS合成缺陷突变体的雄性和雌性性腺中GSC均丢失。dally是一种果蝇磷脂酰肌醇蛋白聚糖，在雌性GSC小生境细胞中表达，并负责维持GSC小生境。dally在卵巢中的异位表达扩大了生态位面积，表明dally是限制GSC生态位空间所必需的。有趣的是，另一个磷脂酰肌醇蛋白聚糖，dally-like，在调节雄性GSC生态位维持中起着重要作用。我们建议，HSPGs定义的物理空间的小生境作为transcoreceptors，介导的分泌因子的短程信号。
Heparan sulfate glycoproteins dally and dally-like define the germ cell niche in female and male Drosophila, respectively. Stem cells are maintained in vivo by short-range signaling systems in specialized microenvironments called niches, but the molecular mechanisms controlling the physical space of the stem cell niche are poorly understood. In this study, we report that heparan sulfate (HS) proteoglycans (HSPGs) are essential regulators of the germline stem cell (GSC) niches in the Drosophila melanogaster gonads. GSCs were lost in both male and female gonads of mutants deficient for HS biosynthesis. dally, a Drosophila glypican, is expressed in the female GSC niche cells and is responsible for maintaining the GSC niche. Ectopic expression of dally in the ovary expanded the niche area, showing that dally is required for restriction of the GSC niche space. Interestingly, the other glypican, dally-like, plays a major role in regulating male GSC niche maintenance. We propose that HSPGs define the physical space of the niche by serving as trans coreceptors, mediating short-range signaling by secreted factors.
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