Moderately increased maternal dietary energy intake delays foetal skeletal muscle differentiation and maturity in pigs
Moderately increased maternal dietary energy intake delays foetal skeletal muscle differentiation and maturity in pigs
复制标题
适度增加母体膳食能量摄入会延迟猪胎儿骨骼肌分化和成熟
DOI：
10.1007/s00394-015-0996-9
复制
发表时间：
2016-06
影响因子：
5
通讯作者：
Chen Daiwen
中科院分区：
文献类型：
--
作者：
Zou Ti;e;He Dongting;Yu Bing;Yu Jie;Mao Xiangbing;Zheng Ping;He Jun;Huang Zhiqing;Shu Yan;Liu Yue;Chen Daiwen
目的以猪为模型，研究妊娠期适度增加母体日粮能量摄入对胎儿骨骼肌发育和代谢的影响。（初始体重135.5 ± 1.6 kg）被分配至两种能量摄入处理之一：正常能量摄入组（Con，30.96 MJ DE/天），如国家研究理事会（NRC; 2012）和高能量摄入组（HE，34.15 MJ DE/天）推荐的。从交配至妊娠第90天引入营养处理。在妊娠第90天，通过背最长肌的形态学、生物化学和分子分析检查胎仔。结果与对照组相比，HE组母猪体重增加（P< 0.05），胎牛肌纤维密度降低（P < 0.05）。同时，HE组胎儿蛋白质浓度、肌酸激酶和乳酸脱氢酶活性及脐静脉血清三碘甲状腺原氨酸（T3）浓度均降低（P< 0.05）。母体高热量饮食降低了肌肉生长相关基因、肌球蛋白重链（MYH/MyHC）基因（MYH 2和MYH 1）、胰岛素样生长因子1和胰岛素生长因子结合蛋白5的mRNA丰度（P< 0.05）。此外，生肌分化因子1、肌细胞生成素和fast-MyHC亚型的蛋白表达在HE胎儿中降低（P< 0.05）。结论我们的结果表明，适度增加母体膳食能量摄入延迟了妊娠90天胎儿骨骼肌的分化和成熟。
ObjectivesThis study aimed to evaluate the effects of moderately increased maternal dietary energy intake during gestation on foetal skeletal muscle development and metabolism with pig as a model.MethodsTwelve primiparous purebred Large White sows (initial body weight 135.5 ± 1.6 kg) were allocated to one of two energy intake treatments: normal-energy-intake group (Con, 30.96 MJ DE/day) as recommended by the National Research Council (NRC; 2012) and high-energy-intake group (HE, 34.15 MJ DE/day). The nutritional treatments were introduced from mating to day 90 of gestation. On day 90 of gestation, foetuses were examined by morphological, biochemical and molecular analysis of the longissimus muscle. Umbilical vein serum hormones were measured.ResultsSow body weight was increased in HE group compared with Con group (P< 0.05), whereas foetal myofibre density was decreased (P< 0.05). Meanwhile, protein concentration, creatine kinase and lactate dehydrogenase activities and umbilical vein serum triiodothyronine (T3) concentration were decreased in HE foetuses (P< 0.05). Maternal HE diets decreased the mRNA abundance of muscle growth-related genes, myosin heavy-chain (MYH/MyHC) genes (MYH2 and MYH1) and insulin-like growth factor 1 and insulin growth factor-binding protein 5 (P< 0.05). Furthermore, the protein expressions of myogenic differentiation factor 1, myogenin and fast-MyHC isoforms were reduced in HE foetuses (P< 0.05).ConclusionOur results suggest that moderately increased maternal dietary energy intake delays the differentiation and maturation in skeletal muscle of the foetus on day 90 of gestation.
登录
查看更多内容
饮食能量密度与美国成年人的肥胖症和其他慢性疾病的生物标志物有关。
影响因子：
5
作者：
Vernarelli JA;Mitchell DC;Rolls BJ;Hartman TJ
通讯作者：
Hartman TJ
影响因子：
2.4
作者：
P. Wigmore;N. Stickland
通讯作者：
P. Wigmore;N. Stickland
DOI：
10.1095/biolreprod.110.089649
发表时间：
2011-07
期刊：
--
影响因子：
--
作者：
Xu Yan;Yan Huang;Junxing Zhao;N. Long;A. B. Uthlaut;Mei J. Zhu;S. Ford;P. Nathanielsz;M. Du
通讯作者：
Xu Yan;Yan Huang;Junxing Zhao;N. Long;A. B. Uthlaut;Mei J. Zhu;S. Ford;P. Nathanielsz;M. Du
影响因子：
5.3
作者：
Jiuqiao Zhao;Schmieg Fi;D. Simmons;G. Molloy
通讯作者：
Jiuqiao Zhao;Schmieg Fi;D. Simmons;G. Molloy
影响因子：
3.6
作者：
C. Rehfeldt;G. Kuhn;J. Vanselow;R. Fürbass;I. Fiedler;G. Nürnberg;A. Clelland;N. Stickland;K. Ender
通讯作者：
C. Rehfeldt;G. Kuhn;J. Vanselow;R. Fürbass;I. Fiedler;G. Nürnberg;A. Clelland;N. Stickland;K. Ender