Increased cortical extracellular adenosine correlates with seizure termination.
Increased cortical extracellular adenosine correlates with seizure termination.
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作者：
Van Gompel JJ;Bower MR;Worrell GA;Stead M;Chang SY;Goerss SJ;Kim I;Bennet KE;Meyer FB;Marsh WR;Blaha CD;Lee KH
癫痫发作目前是根据其电图特征来定义的。然而，神经元网络本质上依赖于神经递质，其中关于其发作期动力学知之甚少。证据支持腺苷在癫痫发作终止中具有突出作用，因为其施用可以终止和减少动物模型中的癫痫发作。此外，人体微透析研究表明腺苷在发作周围升高，但与癫痫发作的关系因其时间测量限制而模糊。由于电化学技术可以提供非常优越的上级时间分辨率，我们测试的假设，细胞外腺苷浓度上升，在癫痫发作终止在动物模型和人类使用电化学。白色农场猪（n=45）被用于癫痫的急性皮质模型和10人癫痫患者进行了研究，在术中皮层脑电图（Ecog）。基于无线瞬时神经递质浓度传感器（WINCS）的快速扫描循环伏安法（FSCV）和固定电位电流法分别利用腺苷特异性三角波或生物传感器获得。同步Ecog和电化学显示，在电描记癫痫发作终止前，与基线相比，在7.5 ± 16.9秒（安培法）（n=75起事件）和2.6 ± 11.2秒（FSCV）（n=15起事件）时腺苷平均升高260%。与这些动物数据一致，还观察到使用FSCV的人类患者在癫痫发作终止前腺苷升高。同步Ecog和电化学记录支持腺苷在癫痫发作终止前升高的假设，表明腺苷本身可能是癫痫发作终止的原因。未来的工作使用术中WINCS基于FSCV记录可能有助于阐明腺苷和癫痫发作终止之间的确切关系。
Seizures are currently defined by their electrographic features. However, neuronal networks are intrinsically dependent upon neurotransmitters of which little is known regarding their peri-ictal dynamics. Evidence supports adenosine as having a prominent role in seizure termination, as its administration can terminate and reduce seizures in animal models. Further, microdialysis studies in humans suggest adenosine is elevated peri-ictally, but the relationship to the seizure is obscured by its temporal measurement limitations. Because electrochemical techniques can provide vastly superior temporal resolution, we test the hypothesis that extracellular adenosine concentrations rise during seizure termination in an animal model and humans using electrochemistry. White farm swine (n=45) were used in an acute cortical model of epilepsy and 10 human epilepsy patients were studied during intraoperative electrocorticography (Ecog). Wireless Instantaneous Neurotransmitter Concentration Sensor (WINCS) based fast scan cyclic voltametry (FSCV) and fixed potential amperometry were obtained utilizing an adenosine specific triangular waveform or biosensors respectively. Simultaneous Ecog and electrochemistry demonstrated an average adenosine rise of 260% compared to baseline at 7.5 ± 16.9 seconds with amperometry (n=75 events) and 2.6 ± 11.2 seconds with FSCV (n=15 events) prior to electrographic seizure termination. In agreement with these animal data, adenosine elevation prior to seizure termination in a human patient utilizing FSCV was also seen. Simultaneous Ecog and electrochemical recording supports the hypothesis that adenosine rises prior to seizure termination, suggesting that adenosine itself may be responsible for seizure termination. Future work using intraoperative WINCS based FSCV recording may help to elucidate the precise relationship between adenosine and seizure termination.
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影响因子：
4.1
作者：
Bledsoe JM;Kimble CJ;Covey DP;Blaha CD;Agnesi F;Mohseni P;Whitlock S;Johnson DM;Horne A;Bennet KE;Lee KH;Garris PA
通讯作者：
Garris PA
影响因子：
5.6
作者：
Boison, D;Huber, A;Möhler, H
通讯作者：
Möhler, H
影响因子：
8.9
作者：
Chang, Su-Youne;Kim, Inyong;Lee, Kendall H.
通讯作者：
Lee, Kendall H.
影响因子：
2.9
作者：
Stoeckler, JD;Poirot, AF;Erion, MD
通讯作者：
Erion, MD
影响因子：
11.2
作者：
DURING, MJ;SPENCER, DD
通讯作者：
SPENCER, DD