Role of serotonergic neurons in the Drosophila larval response to light.
Role of serotonergic neurons in the Drosophila larval response to light.
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DOI：
10.1186/1471-2202-10-66
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发表时间：
2009-06-23
期刊：
影响因子：
2.4
通讯作者：
Campos AR
中科院分区：
文献类型：
--
作者：
Rodriguez Moncalvo VG;Campos AR
果蝇幼虫的运动包括向前蠕动，间歇性的停顿、转身和探索性行为(摇头)。这种行为可以通过视觉输入来调节，表现为光诱导的暂停、头部摆动和方向变化的增加以及以幼虫畏光反应为特征的线速度的降低。在3龄阶段，果蝇幼虫逐渐不再被光排斥，当它们四处游荡寻找变态的地方时，它们是光中和的。因此，果蝇幼虫的光行为可以用来研究运动的控制。我们使用有针对性的神经元沉默来评估候选神经元在调节幼虫光行为中的作用。DOPA脱羧酶(DDC)神经元的失活增加了幼虫整个发育过程中对光的反应，包括以光中和反应为特征的3龄后期。对光的反应增强的特征是光诱导的方向改变和相关停顿的增加，以及线性运动的减少。在DDC神经元中，抑制冠状核能和5-羟色胺能神经元的活动，但不抑制多巴胺能神经元的活动，增加了在3龄时观察到的畏光反应。5-羟色胺能神经元的沉默不会扰乱幼虫的运动或对机械刺激的反应。5-羟色胺能神经元内5-羟色胺(5-羟色胺，5-羟色胺)信号的减少重述了定向神经元沉默所获得的结果。去除腹神经索(VNC)中的5-羟色胺能细胞不会影响幼虫对光的反应。同样，破坏接触大脑半球光感受器终末的5-羟色胺能投射不会影响幼虫对光的反应。最后，泛神经过度表达5-HT1ADro受体，而不是任何其他5-HT1ADro受体亚型，导致3龄幼虫对光的反应显著降低。我们的数据表明，5-羟色胺能神经元和冠状核能神经元的活动有助于控制光对幼虫的运动。我们的结论是，这种控制是由位于大脑半球的5-羟色胺神经元完成的，但似乎不是在光感受器水平上发生的，可能是由5-HT1ADro受体介导的。这些发现为了解5-羟色胺神经元在果蝇幼虫行为中的功能以及调节幼虫对光的反应的机制提供了新的见解。
Drosophila larval locomotion consists of forward peristalsis interrupted by episodes of pausing, turning and exploratory behavior (head swinging). This behavior can be regulated by visual input as seen by light-induced increase in pausing, head swinging and direction change as well as reduction of linear speed that characterizes the larval photophobic response. During 3rd instar stage, Drosophila larvae gradually cease to be repelled by light and are photoneutral by the time they wander in search for a place to undergo metamorphosis. Thus, Drosophila larval photobehavior can be used to study control of locomotion. We used targeted neuronal silencing to assess the role of candidate neurons in the regulation of larval photobehavior. Inactivation of DOPA decarboxylase (Ddc) neurons increases the response to light throughout larval development, including during the later stages of the 3rd instar characterized by photoneutral response. Increased response to light is characterized by increase in light-induced direction change and associated pause, and reduction of linear movement. Amongst Ddc neurons, suppression of the activity of corazonergic and serotonergic but not dopaminergic neurons increases the photophobic response observed during 3rd instar stage. Silencing of serotonergic neurons does not disrupt larval locomotion or the response to mechanical stimuli. Reduced serotonin (5-hydroxytryptamine, 5-HT) signaling within serotonergic neurons recapitulates the results obtained with targeted neuronal silencing. Ablation of serotonergic cells in the ventral nerve cord (VNC) does not affect the larval response to light. Similarly, disruption of serotonergic projections that contact the photoreceptor termini in the brain hemispheres does not impact the larval response to light. Finally, pan-neural over-expression of 5-HT1ADro receptors, but not of any other 5-HT receptor subtype, causes a significant decrease in the response to light of 3rd instar larvae. Our data demonstrate that activity of serotonergic and corazonergic neurons contribute to the control of larval locomotion by light. We conclude that this control is carried out by 5-HT neurons located in the brain hemispheres, but does not appear to occur at the photoreceptor level and may be mediated by 5-HT1ADro receptors. These findings provide new insights into the function of 5-HT neurons in Drosophila larval behavior as well as into the mechanisms underlying regulation of larval response to light.
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DOI：
10.1002/neu.480280305
发表时间：
1995-11-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CAMPOS, AR;LEE, KJ;STELLER, H
通讯作者：
STELLER, H
影响因子：
2.5
作者：
Hassan, J;Iyengar, B;Campos, R
通讯作者：
Campos, R
影响因子：
2.7
作者：
Chen, John;Condron, Barry G.
通讯作者：
Condron, Barry G.
影响因子：
2.9
作者：
Dasari, S;Cooper, RL
通讯作者：
Cooper, RL
影响因子：
4.6
作者：
Choi, Youn-Jeong;Lee, Gyunghee;Park, Jae H.
通讯作者：
Park, Jae H.