Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro.
Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro.
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DOI：
10.1186/1472-6882-14-29
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发表时间：
2014-01-17
影响因子：
--
通讯作者：
Shintani S
中科院分区：
文献类型：
--
作者：
Mukudai Y;Kondo S;Koyama T;Li C;Banka S;Kogure A;Yazawa K;Shintani S
骨质疏松症(OP)是现代世界最严重的疾病之一，OP患者经常患有髋部、脊柱和手腕的脆性骨折，导致生活质量受到限制。虽然双磷酸盐(BPS)是目前可用的最有效的抗骨吸收药物，也是临床上治疗OP最常用的处方，但它们会引起严重的副作用，如双磷酸盐相关性颌骨坏死。因此，可以取代BPS使用的新型治疗材料已经被开发出来。我们启动了一个机构合作项目，从400多种具有生物活性的草药产品中挑选出候选草药提取物，不仅对OP有潜在的治疗效果，而且对口腔和骨骼疾病也有潜在的疗效。本文报道了苦荞、延胡索和白首乌3种中草药的树皮提取物。这些提取物均通过上调caspase活性和增加线粒体促凋亡蛋白的表达来抑制破骨细胞的增殖和诱导细胞凋亡。此外，提取物促进了成骨细胞和软骨细胞的分化，但不影响其增殖。在原代培养的骨髓细胞中也观察到了骨诱导效应。虽然这些提取物在传统中医中的应用已有数百年的历史，但据我们所知，它们对OP的疗效尚未见报道。在这项研究中，我们阐明了这些草药提取物作为OP治疗的新候选药物的有效性。
Osteoporosis (OP) is one of the most serious diseases in the modern world, and OP patients frequently suffer from fragility fractures in the hip, spine and wrist, resulting in a limited quality of life. Although bisphosphonates (BPs) are the most effective class of anti-bone-resorptive drugs currently available and the most commonly prescribed for the clinical treatment of OP, they are known to cause serious side effects such as bisphosphonate-related osteonecrosis of the jaw. Novel therapeutic materials that can replace the use of BPs have therefore been developed. We commenced an institutional collaborative project in which candidates of herbal extracts were selected from more than 400 bioactive herbal products for their potential therapeutic effects not only in OP, but also in oral and skeletal diseases. In the present study, we report on 3 Chinese medical herbal extracts from the root barks of Melia azedarach, Corydalis turtschaninovii, and Cynanchum atratum. All of these extracts inhibited osteoclast proliferation and induced apoptosis by up-regulation of caspase activity and increase of mitochondrial pro-apoptotic proteins expression. Furthermore, the extracts enhanced differentiation, but did not affect proliferation of both osteoblasts and chondrocytes. The osteo-inducible effect was also observed in cultured primary bone marrow cells. Although these extracts have been utilized in traditional Chinese medicine for hundreds of years, there are no reports to our knowledge, on their therapeutic effects in OP. In this study, we elucidate the potency of these herbal extracts as novel candidates for OP therapy.
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影响因子：
4.4
作者：
Bharti, AC;Takada, Y;Aggarwal, BB
通讯作者：
Aggarwal, BB
DOI：
10.1083/jcb.140.2.409
发表时间：
1998-01-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Enomoto-Iwamoto M;Iwamoto M;Mukudai Y;Kawakami Y;Nohno T;Higuchi Y;Takemoto S;Ohuchi H;Noji S;Kurisu K
通讯作者：
Kurisu K
影响因子：
5.1
作者：
Day, SH;Wang, JP;Lin, CN
通讯作者：
Lin, CN
影响因子：
3.3
作者：
Yoneda, Toshiyuki;Hagino, Hiroshi;Urade, Masahiro
通讯作者：
Urade, Masahiro
影响因子：
--
作者：
Guo, Chun;Hou, Guo-qing;Du, Shi-xin
通讯作者：
Du, Shi-xin