Broad phylogeny and functionality of cellulosomal components in the bovine rumen microbiome.
Broad phylogeny and functionality of cellulosomal components in the bovine rumen microbiome.
复制标题
牛瘤胃微生物组中纤维体成分的宽大系统发育和功能。
DOI：
10.1111/1462-2920.13561
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发表时间：
2017-01
影响因子：
5.1
通讯作者：
Mizrahi I
中科院分区：
文献类型：
--
作者：
Bensoussan L;Moraïs S;Dassa B;Friedman N;Henrissat B;Lombard V;Bayer EA;Mizrahi I
纤维素体是一种细胞外多酶复合物，被认为是自然界设计的最有效的植物细胞壁降解策略之一。其独特的模块化架构通过蛋白质模块（粘连蛋白和坞蛋白）之间的高亲和力和特异性相互作用实现，能够形成各种酶组合。人们对纤维素体复合物的机械性质进行了广泛的研究。然而，人们对其在富含天然植物纤维的环境中的微生物中的分布和丰度知之甚少。在这里，我们在牛瘤胃微生物群落中探索了这些问题，专门从事木质纤维素植物材料的有效降解。我们使用先前发表的超深纤维粘附瘤胃宏基因组，通过生物信息学方法筛选了这种复杂环境中的纤维素体模块。有趣的是，含有码头蛋白的蛋白质的大部分功能与替代生物过程有关，而不一定与经典的纤维降解功能有关。我们的分析通过表征选定的粘连蛋白和坞蛋白之间的特定相互作用进行了实验验证，并揭示了纤维素体是多种细菌使用的更通用的策略，其中一些细菌以前与纤维素体产生无关。值得注意的是，我们的结果提供了全球瘤胃微生物群落相似性的额外证据。这项研究表明纤维素体机制在自然界中具有更广泛和广泛的作用。
The cellulosome is an extracellular multi‐enzyme complex that is considered one of the most efficient plant cell wall‐degrading strategies devised by nature. Its unique modular architecture, achieved by high affinity and specific interaction between protein modules (cohesins and dockerins) enables formation of various enzyme combinations. Extensive research has been dedicated to the mechanistic nature of the cellulosome complex. Nevertheless, little is known regarding its distribution and abundance among microbes in natural plant fibre‐rich environments. Here, we explored these questions in bovine rumen microbial communities, specialized in efficient degradation of lignocellulosic plant material. We bioinformatically screened for cellulosomal modules in this complex environment using a previously published ultra‐deep fibre‐adherent rumen metagenome. Intriguingly, a large portion of the functions of the dockerin‐containing proteins were related to alternative biological processes, and not necessarily to the classic fibre degradation function. Our analysis was experimentally validated by characterizing specific interactions between selected cohesins and dockerins and revealed that cellulosome is a more generalized strategy used by diverse bacteria, some of which were not previously associated with cellulosome production. Remarkably, our results provide additional proof of similarity among rumen microbial communities worldwide. This study suggests a broader and widespread role for the cellulosomal machinery in nature.
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影响因子：
17.3
作者：
BAYER, EA;MORAG, E;LAMED, R
通讯作者：
LAMED, R
影响因子：
4.4
作者：
Caspi, Jonathan;Barak, Yoav;Bayer, Edward A.
通讯作者：
Bayer, Edward A.
影响因子：
2.3
作者：
Jami, Elie;Mizrahi, Itzhak
通讯作者：
Mizrahi, Itzhak
影响因子：
3.7
作者：
Jami E;Mizrahi I
通讯作者：
Mizrahi I
影响因子：
4.8
作者：
Fierobe, HP;Mingardon, F;Bayer, EA
通讯作者：
Bayer, EA