GONST2 transports GDP-Mannose for sphingolipid glycosylation in the Golgi apparatus of Arabidopsis
GONST2 transports GDP-Mannose for sphingolipid glycosylation in the Golgi apparatus of Arabidopsis
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GONST2 在拟南芥高尔基体中转运 GDP-甘露糖进行鞘脂糖基化
DOI：
10.1101/346775
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jing B
中科院分区：
文献类型：
--
作者：
Jing B
The Golgi lumen is the site of many different glycosylation events, including cell wall polysaccharide biosynthesis and lipid glycosylation. Transporters are necessary for the import of the substrates required for glycosylation (nucleotide sugars) from the cytosol where they are synthesized. Plants use four GDP-linked sugars to glycosylate macromolecules: GDP-L-Fucose, GDP-D-Mannose, GDP-L-Galactose and GDP-D-Glucose. Of the predicted fifty-one members of the nucleotide sugar transporter/triose phosphate transporter family in Arabidopsis, only four appear to contain the conserved motif needed for the transport of GDP-linked sugars, GOLGI LOCALIZED NUCLEOTIDE SUGAR TRANSPORTER (GONST) 1-4. Previously, we have demonstrated that GONST1 provides GDP-D-Mannose for glycosylation of a class of sphingolipids, the glycosylinositolphosphorylceramides (GIPCs). Here, we characterize its closest homologue, GONST2, and conclude that it also specifically provides substrate for GIPC glycosylation. Expression ofGONST2driven by theGONST1promoter is able to rescue the severe growth phenotype ofgonst1. Loss of GONST2 exacerbates thegonst1constitutive hypersensitive response, as well as the reduced cell wall cellulose content. Thegonst2mutant grows normally under standard conditions, but has enhanced resistance to the powdery mildew-causing fungusGolovinomyces orontii.
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影响因子：
2.9
作者：
KAUL, K;LESTER, RL
通讯作者：
LESTER, RL
影响因子：
2.9
作者：
HSIEH, TCY;KAUL, K;LESTER, RL
通讯作者：
LESTER, RL
比较植物鞘脂组揭示了种子和油中的特定脂质。
影响因子：
3.8
作者：
Frédérique Tellier;A. Maia;I. Schmitz;J. Faure
通讯作者：
J. Faure
[47] 中性鞘糖脂的薄层色谱法
DOI：
--
发表时间：
1975
期刊：
影响因子：
--
作者：
V. P. Skipski
通讯作者：
V. P. Skipski
影响因子：
3.8
作者：
Cacas, Jean-Luc;Bure, Corinne;Mongrand, Sebastien
通讯作者：
Mongrand, Sebastien