Self-assembly and elasticity of hierarchical proteoglycan–hyaluronan brushes †Electronic supplementary information (ESI) available: Variations in areal mass density upon SLB and SAv monolayer formation determined by SE (Fig. S1). See DOI: 10.1039/c3sm51213d Click here for additional data file.
Self-assembly and elasticity of hierarchical proteoglycan–hyaluronan brushes †Electronic supplementary information (ESI) available: Variations in areal mass density upon SLB and SAv monolayer formation determined by SE (Fig. S1). See DOI: 10.1039/c3sm51213d Click here for additional data file.
复制标题
DOI：
10.1039/c3sm51213d
复制
发表时间：
2013-07-22
期刊：
影响因子：
3.4
通讯作者：
Richter RP
中科院分区：
文献类型：
--
作者：
Attili S;Richter RP
我们组装了含有聚集素的透明质酸刷子，以研究富含透明质酸的细胞外基质中超分子结构和动力学与材料性质的关系。由蛋白多糖和透明质酸(HA)形成的空间受限但强烈水化的组合体是软骨细胞周围细胞周围空间和软骨中主要的、功能重要的组成部分。为了更好地理解这种组件的超分子结构和动力学是如何产生机械性能的，我们研究了聚集聚糖对定义明确的平面HA刷子的物理化学性能的影响。通过石英晶体微天平与耗散监测和椭圆偏振光谱的相互作用研究，以及胶体探针原子力/反射干涉对比显微镜的压缩研究，我们发现aggrecan很容易以可逆的方式嵌入到HA刷子中。Aggrecan会导致HA刷子的急剧膨胀，产生几微米厚的高度水合(>；99%)、弹性和非常柔软的自组装膜。在线性压缩条件下，弹性模数远低于100Pa.在强压缩条件下，杨氏模数可达数kPa.讨论了这些发现对生物功能的影响。
We assemble aggrecan-containing hyaluronan brushes to study how the supramolecular structure and dynamics relate to material properties in hyaluronan-rich pericellular matrices. Spatially confined yet strongly hydrated assemblies made from the proteoglycan aggrecan and the polysaccharide hyaluronan (HA) are major, functionally important components of the pericellular space around chondrocytes, and in cartilage. To better understand, how mechanical properties arise from the supramolecular structure and dynamics of such assemblies, we have studied the effect of aggrecan on the physico-chemical properties of well-defined, planar HA brushes. From interaction studies by quartz crystal microbalance with dissipation monitoring and spectroscopic ellipsometry, and compression studies by combined colloidal probe atomic force/reflection interference contrast microscopy, we find that aggrecan readily intercalates into HA brushes in a reversible manner. Aggrecan induces a drastic swelling of HA brushes, generating self-assembled films of several micrometers in thickness that are highly hydrated (>99%), elastic and very soft. The Young modulus in the linear compression regime is well below 100 Pa, and reaches several kPa at strong compression. The implications of these findings for biological function are discussed.
登录
查看更多内容
影响因子：
3.2
作者：
Cohen, M;Joester, D;Addadi, L
通讯作者：
Addadi, L
影响因子：
2.4
作者：
Dean, Delphine;Han, Lin;Ortiz, Christine
通讯作者：
Ortiz, Christine
影响因子：
8.6
作者：
BINNIG, G;QUATE, CF;GERBER, C
通讯作者：
GERBER, C
影响因子：
15.6
作者：
DEGENNES, PG
通讯作者：
DEGENNES, PG
影响因子：
3.4
作者：
Boehm, Heike;Mundinger, Tabea A.;Curtis, Jennifer E.
通讯作者：
Curtis, Jennifer E.