Early IFN-gamma production and innate immunity during Listeria monocytogenes infection in the absence of NK cells.
Early IFN-gamma production and innate immunity during Listeria monocytogenes infection in the absence of NK cells.
复制标题
在缺乏 NK 细胞的情况下，单核细胞增生李斯特菌感染期间的早期 IFN-γ 产生和先天免疫。
作者：
Åsa Andersson;W. J. Dai;J. D. Di Santo;F. Brombacher
NK细胞被认为在单核细胞增生李斯特菌感染的早期阶段通过产生IFN-γ发挥强制性作用，IFN-γ是激活巨噬细胞效应子功能所需的。缺乏常见细胞因子受体γ链（γ（c）-/-）的小鼠完全缺乏NK细胞，用于检查NK细胞是否是体内抵抗李斯特菌感染所必需的。令人惊讶的是，感染的γ（c）-/-小鼠在感染后2天表现出正常的先天免疫和巨噬细胞对亚致死性李斯特菌感染的应答。在这个时间点，与未感染的对照组相比，γ（c）-/-小鼠显示出增加的血液IFN-γ水平，证明了IFN-γ的非NK依赖性来源，这解释了早期抗性。李斯特菌感染的γ（c）-/- x重组酶激活基因-2-/-双缺陷小鼠不能产生IFN-γ，对李斯特菌高度敏感。单核细胞增多症。由于T细胞而不是B细胞是主要的IFN-γ产生者，并且发现γ（c）-/- T细胞在体外是有效的IFN-γ产生者，因此我们从这些结果得出结论，T细胞在功能上替代NK细胞进行早期IFN-γ产生，这是在感染L.单核细胞增多症这种新的观察强调了适应性免疫反应如何维持和影响先天免疫。
NK cells are believed to play a mandatory role during the early phases of Listeria monocytogenes infection by producing IFN-gamma, which is required for the activation of macrophage effector functions. Mice deficient in the common cytokine receptor gamma-chain (gamma(c)-/-), which completely lack NK cells, were used to examine whether NK cells were essential for resistance to Listeria infection in vivo. Surprisingly, infected gamma(c)-/- mice showed normal innate immunity and macrophage responses against sublethal Listeria infection 2 days postinfection. At this time point, gamma(c)-/- mice showed increased blood IFN-gamma levels compared with those in noninfected controls, demonstrating an NK-independent source of IFN-gamma, which explains early resistance. Listeria-infected gamma(c)-/- x recombinase-activating gene-2-/- double-deficient mice were unable to produce IFN-gamma and were highly susceptible to L. monocytogenes. Since T cells, but not B cells, are major IFN-gamma producers, and gamma(c)-/- T cells were found to be efficient IFN-gamma producers in vitro, we conclude from these results that T cells functionally replace NK cells for the early IFN-gamma production that is necessary for activating the innate immune system following infection with L. monocytogenes. This novel observation in listeriosis underscores how the adaptive immune response can maintain and influence innate immunity.
影响因子：
56.9
作者：
HUANG, S;HENDRIKS, W;AGUET, M
通讯作者：
AGUET, M
影响因子：
32.4
作者：
WILLERFORD, DM;CHEN, JZ;ALT, FW
通讯作者：
ALT, FW