Maternal metabolism affects endometrial expression of oxidative stress and FOXL2 genes in cattle.
Maternal metabolism affects endometrial expression of oxidative stress and FOXL2 genes in cattle.
复制标题
DOI：
10.1371/journal.pone.0189942
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sandra O
中科院分区：
文献类型：
--
作者：
Lesage-Padilla A;Forde N;Poirée M;Healey GD;Giraud-Delville C;Reinaud P;Eozenou C;Vitorino Carvalho A;Galio L;Raliou M;Oudin JF;Richard C;Sheldon IM;Charpigny G;Lonergan P;Sandra O
对产奶量的密集选择导致高产奶牛的繁殖效率降低。密集产奶对卵母细胞质量以及早期胚胎发育的影响已经确定，但很少有分析在植入开始时解决这个问题，这是确保成功怀孕和正常产后发育的关键里程碑。我们的研究旨在确定对比母体代谢是否影响先前描述的牛子宫内膜对孕体的感觉特性。在发情后第7天的胚胎移植后，在第19天从分娩后立即干燥的经产产后Holstein-Friesian母牛收集子宫内膜caruncular（CAR）和caruncular间（ICAR）区域（即，从不挤奶; DRY）或每天挤奶两次（LACT）。基因定量表明，哺乳对先前报道为孕体调节（PLET 1，PTGS 2，SOCS 6）和干扰素-tau刺激（RSAD 2，SOCS 1，SOCS 3，STAT 1）因子或已知为女性子宫内膜调节基因（FOXL 2，SCARA 5，PTGS 2）的转录物的子宫内膜表达没有显著影响。与LACT奶牛相比，DRY奶牛表现出FOXL 2转录因子mRNA水平的表达增加和氧化应激相关基因（CAT，SOD 1，SOD 2）的表达降低。在体内和体外实验中强调，无论是干扰素-tau或FOXL 2都不参与CAT，SOD 1和SOD 2的转录调控。此外，我们的数据表明，母体代谢的变化对ICAR区域的基因表达有更大的影响。总的来说，我们的研究结果提示，需要充分了解子宫内膜生理学的变化在多大程度上推动了孕体发育的轨迹，从植入开始，当母体代谢改变。
Intensive selection for milk production has led to reduced reproductive efficiency in high-producing dairy cattle. The impact of intensive milk production on oocyte quality as well as early embryo development has been established but few analyses have addressed this question at the initiation of implantation, a critical milestone ensuring a successful pregnancy and normal post-natal development. Our study aimed to determine if contrasted maternal metabolism affects the previously described sensory properties of the endometrium to the conceptus in cattle. Following embryo transfer at Day 7 post-oestrus, endometrial caruncular (CAR) and intercaruncular (ICAR) areas were collected at Day 19 from primiparous postpartum Holstein-Friesian cows that were dried-off immediately after parturition (i.e., never milked; DRY) or milked twice daily (LACT). Gene quantification indicated no significant impact of lactation on endometrial expression of transcripts previously reported as conceptus-regulated (PLET1, PTGS2, SOCS6) and interferon-tau stimulated (RSAD2, SOCS1, SOCS3, STAT1) factors or known as female hormone-regulated genes (FOXL2, SCARA5, PTGS2). Compared with LACT cows, DRY cows exhibited mRNA levels with increased expression for FOXL2 transcription factor and decreased expression for oxidative stress-related genes (CAT, SOD1, SOD2). In vivo and in vitro experiments highlighted that neither interferon-tau nor FOXL2 were involved in transcriptional regulation of CAT, SOD1 and SOD2. In addition, our data showed that variations in maternal metabolism had a higher impact on gene expression in ICAR areas. Collectively, our findings prompt the need to fully understand the extent to which modifications in endometrial physiology drive the trajectory of conceptus development from implantation onwards when maternal metabolism is altered.
登录
查看更多内容
泌乳和妊娠对荷斯坦母牛子宫内膜基因表达的影响在发情周期或妊娠的第17天。
影响因子：
3.5
作者：
Cerri RL;Thompson IM;Kim IH;Ealy AD;Hansen PJ;Staples CR;Li JL;Santos JE;Thatcher WW
通讯作者：
Thatcher WW
影响因子：
3.6
作者：
Eozenou, Caroline;Carvalho, Anais Vitorino;Sandra, Olivier
通讯作者：
Sandra, Olivier
影响因子：
3.6
作者：
Cronin JG;Turner ML;Goetze L;Bryant CE;Sheldon IM
通讯作者：
Sheldon IM
影响因子：
3.5
作者：
Collard, BL;Boettcher, PJ;Schaeffer, LR
通讯作者：
Schaeffer, LR
影响因子：
2.8
作者：
Al-Katanani, YM;Drost, M;Hansen, PJ
通讯作者：
Hansen, PJ