The chemokine receptor CX(3)CR1 coordinates monocyte recruitment and endothelial regeneration after arterial injury.
The chemokine receptor CX(3)CR1 coordinates monocyte recruitment and endothelial regeneration after arterial injury.
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DOI：
10.15252/emmm.201707502
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发表时间：
2018-03
影响因子：
11.1
通讯作者：
Limbourg FP
中科院分区：
文献类型：
--
作者：
Getzin T;Krishnasamy K;Gamrekelashvili J;Kapanadze T;Limbourg A;Häger C;Napp LC;Bauersachs J;Haller H;Limbourg FP
损伤后动脉内皮的再生对于维持正常的血流、细胞运输和血管功能至关重要。通过小鼠颈动脉损伤模型，我们发现内皮再生从静态到动态阶段的转变是以内皮细胞增殖的强烈增加为标志的，内皮细胞增殖伴随着伤口边缘内皮细胞中趋化因子cx3 CL1的诱导，导致Ly6Clo单核细胞的逐渐募集，表达高水平的同源cx3 CR1趋化因子受体。在Cx3cr1缺失的小鼠中，Ly6Clo单核细胞的募集，颈动脉损伤后内皮细胞的增殖和内皮单层的再生受到损害，这可以通过正常Ly6Clo单核细胞的急性转移来挽救。此外，在共培养实验中，人类非经典单核细胞以VEGFA依赖的方式诱导内皮细胞增殖，并且在体内杂交小鼠模型中，颈动脉损伤后单核细胞转移促进内皮细胞伤口愈合。因此，cx3 CR1协调特定单核细胞亚群募集到内皮再生部位，从而促进内皮细胞增殖和动脉再生。
Regeneration of arterial endothelium after injury is critical for the maintenance of normal blood flow, cell trafficking, and vascular function. Using mouse models of carotid injury, we show that the transition from a static to a dynamic phase of endothelial regeneration is marked by a strong increase in endothelial proliferation, which is accompanied by induction of the chemokine CX 3 CL1 in endothelial cells near the wound edge, leading to progressive recruitment of Ly6Clo monocytes expressing high levels of the cognate CX 3 CR1 chemokine receptor. In Cx3cr1‐deficient mice recruitment of Ly6Clo monocytes, endothelial proliferation and regeneration of the endothelial monolayer after carotid injury are impaired, which is rescued by acute transfer of normal Ly6Clo monocytes. Furthermore, human non‐classical monocytes induce proliferation of endothelial cells in co‐culture experiments in a VEGFA‐dependent manner, and monocyte transfer following carotid injury promotes endothelial wound closure in a hybrid mouse model in vivo. Thus, CX 3 CR1 coordinates recruitment of specific monocyte subsets to sites of endothelial regeneration, which promote endothelial proliferation and arterial regeneration.
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影响因子：
8.7
作者：
Chen, DH;Walsh, K;Wang, J
通讯作者：
Wang, J
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR
影响因子：
3.5
作者：
Becher, Ulrich M.;Moeller, Lisa;Werner, Nikos
通讯作者：
Werner, Nikos
在动脉粥样硬化之前和开发过程中淋巴细胞募集到主动脉壁是部分依赖性L-选择蛋白。
DOI：
10.1084/jem.20052205
发表时间：
2006-05-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
20.1
作者：
Gimbrone MA Jr;García-Cardeña G
通讯作者：
García-Cardeña G