Jasmonate inhibits COP1 activity to suppress hypocotyl elongation and promote cotyledon opening in etiolated Arabidopsis seedlings
Jasmonate inhibits COP1 activity to suppress hypocotyl elongation and promote cotyledon opening in etiolated Arabidopsis seedlings
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茉莉酸抑制 COP1 活性，抑制黄化拟南芥幼苗下胚轴伸长并促进子叶张开
DOI：
10.1111/tpj.13539
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发表时间：
2017-06
期刊：
影响因子：
7.2
通讯作者：
Zhu Ziqiang
中科院分区：
文献类型：
--
作者：
Zheng Yuyu;Cui Xuefei;Su Liang;Fang Shuang;Chu Jinfang;Gong Qingqiu;Yang Jianping;Zhu Ziqiang
发芽的幼苗经历了形态发生，从土壤中出来，走向光明。在这一阶段，子叶闭合，下胚轴伸长。在光照下，幼苗通过打开子叶和抑制下胚轴伸长而迅速转向光形态发生。E3泛素连接酶组成型光形态形成1 （COP1）对维持脑形态形成至关重要。在这里，我们报道茉莉酸（JA）抑制黄化幼苗的下胚轴伸长并刺激子叶开放，在黑暗中部分表型复制cop1突变体。我们还发现JA以依赖cop1的方式稳定了几种cop1靶向转录因子。RNA-seq分析进一步定义了一个JA-light共调制和cop1依赖的转录组，该转录组富含生长素应答基因和参与细胞壁修饰的基因。JA至少通过两种不同的机制抑制COP1的活性：减少COP1蛋白在细胞核中的积累；并减少COP1与其激活剂——光敏色素a - 1051抑制因子（SPA1）之间的物理相互作用。我们的研究表明，JA抑制COP1活性以稳定COP1靶点，从而抑制黄化拟南芥幼苗的下胚轴伸长并刺激子叶展开。
A germinating seedling undergoes skotomorphogenesis to emerge from the soil and reach for light. During this phase, the cotyledons are closed, and the hypocotyl elongates. Upon exposure to light, the seedling rapidly switches to photomorphogenesis by opening its cotyledons and suppressing hypocotyl elongation. The E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) is critical for maintaining skotomorphogenesis. Here, we report that jasmonate (JA) suppresses hypocotyl elongation and stimulates cotyledon opening in etiolated seedlings, partially phenocopying cop1 mutants in the dark. We also find that JA stabilizes several COP1-targeted transcription factors in a COP1-dependent manner. RNA-seq analysis further defines a JA-light co-modulated and cop1-dependent transcriptome, which is enriched for auxin-responsive genes and genes participating in cell wall modification. JA suppresses COP1 activity through at least two distinct mechanisms: decreasing COP1 protein accumulation in the nucleus; and reducing the physical interaction between COP1 and its activator, SUPPRESSOR OF PHYTOCHROME A-105 1 (SPA1). Our work reveals that JA suppresses COP1 activity to stabilize COP1 targets, thereby inhibiting hypocotyl elongation and stimulating cotyledon unfolding in etiolated Arabidopsis seedlings.
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