Transmission of aerosolized seasonal H1N1 influenza A to ferrets.
Transmission of aerosolized seasonal H1N1 influenza A to ferrets.
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DOI：
10.1371/journal.pone.0024448
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Koster F
中科院分区：
文献类型：
--
作者：
MacInnes H;Zhou Y;Gouveia K;Cromwell J;Lowery K;Layton RC;Zubelewicz M;Sampath R;Hofstadler S;Liu Y;Cheng YS;Koster F
流感病毒是全世界发病率和死亡率的一个主要原因，但对传播的定量了解很少，无法指导以证据为基础的公共卫生实践。最近关于雪貂和豚鼠之间流感非接触传播的研究，为大流行毒株和季节性毒株的相对传播效率提供了见解，但大多数毒株的感染剂量和随后的传染尚未量化。为了测量50%血清阴性雪貂的气溶胶感染剂量（aID50），将季节性流感病毒雾化到暴露室中，控制气流，限制吸入直径小于5µm的空气中颗粒。在不同剂量的雾化病毒雾化过程中，用液体撞击器和特氟龙过滤器收集空气传播的病毒。由于可培养的病毒在过滤器上仅需20分钟即可准确捕获，因此在暴露1小时期间收集的空气传播病毒RNA通过两种分析进行定量，一种是检测6个基因组片段的高通量RT-PCR/质谱分析（Ibis T5000™生物传感器系统），另一种是标准的实时RT-qPCR分析。使用灵敏度更高的T5000检测，在所使用的暴露条件下，A/新喀里多尼亚/20/99 （H1N1）的aID50约为4个感染性病毒颗粒。虽然血清转化和上呼吸道分泌物中病毒RNA的持续水平提示已建立的粘膜感染，但病毒培养几乎总是阴性。因此，在吸入后，这种季节性H1N1病毒在粘膜沉积后的复制效率可能低于H3N2病毒，并且在气溶胶暴露后表现出较少的传染性。
Influenza virus is a major cause of morbidity and mortality worldwide, yet little quantitative understanding of transmission is available to guide evidence-based public health practice. Recent studies of influenza non-contact transmission between ferrets and guinea pigs have provided insights into the relative transmission efficiencies of pandemic and seasonal strains, but the infecting dose and subsequent contagion has not been quantified for most strains. In order to measure the aerosol infectious dose for 50% (aID50) of seronegative ferrets, seasonal influenza virus was nebulized into an exposure chamber with controlled airflow limiting inhalation to airborne particles less than 5 µm diameter. Airborne virus was collected by liquid impinger and Teflon filters during nebulization of varying doses of aerosolized virus. Since culturable virus was accurately captured on filters only up to 20 minutes, airborne viral RNA collected during 1-hour exposures was quantified by two assays, a high-throughput RT-PCR/mass spectrometry assay detecting 6 genome segments (Ibis T5000™ Biosensor system) and a standard real time RT-qPCR assay. Using the more sensitive T5000 assay, the aID50 for A/New Caledonia/20/99 (H1N1) was approximately 4 infectious virus particles under the exposure conditions used. Although seroconversion and sustained levels of viral RNA in upper airway secretions suggested established mucosal infection, viral cultures were almost always negative. Thus after inhalation, this seasonal H1N1 virus may replicate less efficiently than H3N2 virus after mucosal deposition and exhibit less contagion after aerosol exposure.
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影响因子：
5.4
作者：
Mitchell, Hugh;Levin, Drew;Koster, Frederick
通讯作者：
Koster, Frederick
影响因子：
4.8
作者：
Lednicky, John A.;Hamilton, Sara B.;Swayne, David E.
通讯作者：
Swayne, David E.
影响因子：
5.8
作者：
Fabian P;McDevitt JJ;Houseman EA;Milton DK
通讯作者：
Milton DK
DOI：
10.1073/pnas.0409920102
发表时间：
2005-05-31
影响因子：
11.1
作者：
Ecker, DJ;Sampath, R;Hofstadler, SA
通讯作者：
Hofstadler, SA
影响因子：
5.4
作者：
Marcus, Philip I.;Ngunjiri, John M.;Sekellick, Margaret J.
通讯作者：
Sekellick, Margaret J.