L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells
L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells
复制标题
左旋肉碱与牛磺酸协同抑制血管平滑肌细胞增殖和成骨分化
DOI：
10.1038/aps.2009.206
复制
发表时间：
2010-02
影响因子：
8.2
通讯作者：
廖晓波
中科院分区：
文献类型：
--
作者：
廖晓波
目的：探讨左旋卡尼汀（LC）和牛磺酸（TAU）对血管平滑肌细胞（VSMCs）增殖和成骨分化的协同作用。以碱性磷酸酶（ALP）活性和钙含量为指标，观察LC和TAU对VSMCs成骨分化和矿化的影响。测定VSMCs对TAU的摄取。结果：LC和TAU协同抑制VSMCs增殖和β-甘油磷酸（β-GP）诱导的成骨分化，表现为降低[3 H]胸苷掺入、ALP活性和钙沉积。LC还可促进VSMCs对TAU的摄取和TAUT的表达。结论：LC和TAU对VSMCs增殖和成骨分化的协同抑制作用与LC上调TAUT表达和摄取TAU有关。
Aim:To investigate the synergistic action of L-carnitine (LC) and taurine (TAU) on the proliferation and osteoblastic differentiation of vascular smooth muscle cells (VSMCs).Methods:DNA and protein synthesis of VSMCs were assessed using scintillation counting. Alkaline phosphatase (ALP) activity and calcium content were determined to investigate the effects of LC and TAU on the osteoblastic differentiation and mineralization of VSMCs. TAU uptake by VSMCs was assayed. RNA interference was used to down-regulate the expression of the TAU transporter (TAUT) in rat VSMCs.Results:LC and TAU synergistically inhibited the proliferation and β-glycerophosphate (β-GP)-induced osteoblastic differentiation of VSMCs as evidenced by the decreased [3 H] thymidine incorporation, ALP activity and calcium deposition. Furthermore, LC stimulated the TAU uptake and TAUT expression in VSMCs. Suppression of TAUT with short hairpin RNA (shRNA) abolished the synergistic action of LC and TAU in VSMCs.Conclusion:The synergistic inhibitory action of LC and TAU on the proliferation and osteoblastic differentiation of VSMCs is attributable to the up-regulation of TAUT expression and TAU uptake by LC.
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DOI：
10.1016/j.bbamcr.2005.07.005
发表时间：
2005-09
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
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DOI：
--
发表时间：
1960-10
期刊：
The American journal of pathology
影响因子：
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