Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products.
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products.
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开发合成生物学工具，将巴斯德毕赤酵母作为天然产物生产的基础。
DOI：
10.1016/j.synbio.2021.04.005
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发表时间：
2021-06
影响因子：
4.8
通讯作者：
Lian J
中科院分区：
文献类型：
--
作者：
Gao J;Jiang L;Lian J
甲基营养型酵母巴斯德毕赤酵母（Pichia pastoris）（a.k.a. Komagataella phaffii）是工业生产重组蛋白最常用的宿主之一。巴斯德毕赤酵母作为一种非传统酵母，具有独特的生物学特性，其表达系统已经比较成熟。随着合成生物学的发展，人们致力于将巴斯德毕赤酵母开发成生产各种高价值化合物（如天然产物）的基础。本文首先介绍了用于巴斯德毕赤酵母工程改造的合成生物学工具，包括用于异源基因表达的载体、启动子和终止子，以及用于基因组编辑的规则间隔短回文重复序列/CRISPR相关系统（CRISPR/Cas）。本综述随后通过代谢工程巴斯德毕赤酵母菌株生产增值天然产物的实例。最后，展望了毕赤酵母作为合成生物学基础的发展前景。
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P. pastoris has unique biological characteristics and its expression system has been well developed. With the advances in synthetic biology, more efforts have been devoted to developing P. pastoris into a chassis for the production of various high-value compounds, such as natural products. This review begins with the introduction of synthetic biology tools for the engineering of P. pastoris, including vectors, promoters, and terminators for heterologous gene expression as well as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated System (CRISPR/Cas) for genome editing. This review is then followed by examples of the production of value-added natural products in metabolically engineered P. pastoris strains. Finally, challenges and outlooks in developing P. pastoris as a synthetic biology chassis are prospected.
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