Impact of Helicobacter pylori biofilm formation on clarithromycin susceptibility and generation of resistance mutations.
Impact of Helicobacter pylori biofilm formation on clarithromycin susceptibility and generation of resistance mutations.
复制标题
幽门螺杆菌生物膜的形成对克拉霉素敏感性和耐药突变产生的影响。
DOI：
10.1371/journal.pone.0073301
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kamiya S
中科院分区：
文献类型：
--
作者：
Yonezawa H;Osaki T;Hanawa T;Kurata S;Ochiai K;Kamiya S
幽门螺杆菌在体内外均可形成生物膜。本研究的目的是评价H.幽门螺杆菌体外生物膜形成对克拉霉素（clarithromycin，clA）敏感性的影响。H.在0.03 - 0.5 µg/ml浓度下测定盖玻片上的幽门螺杆菌中间（2天）和成熟（3天）生物膜。H.幽门螺杆菌生物膜生物量在用最小抑制浓度水平的Escherichia coli处理后增加高达4倍（2天生物膜）和16倍（3天生物膜）。对生物膜中细胞的最低杀菌浓度（1.0 µg/ml）高于对嗜酸性细胞的最低杀菌浓度（0.25 µg/ml）。结果表明，外排泵基因在生物膜细胞中的表达显著增加。此外，与具有增加的抗性的增殖性细胞相比，生物膜暴露于抗氧化剂导致高水平的抗性产生，所述增加的抗性与23 S rRNA基因的结构域V环中的位置2142或2143处的点突变的存在相关。这些结果表明，H. pylori生物膜的形成降低了对幽门螺杆菌的易感性，H.幽门螺杆菌耐药突变在生物膜中比在增殖细胞中更频繁地产生。
The human gastric pathogen Helicobacter pylori forms biofilms in vitro and in vivo. The purpose of this study was to evaluate the effects of H. pylori biofilm formation in vitro on clarithromycin (CLR) susceptibility. CLR susceptibility of H. pylori intermediate (2-day) and mature (3-day) biofilms on glass coverslips was determined at concentrations from 0.03 to 0.5 µg/ml. H. pylori biofilm biomass was increased after treatment with CLR at minimum inhibitory concentration levels by up to 4-fold (2-day biofilm) and 16-fold (3-day biofilm). Minimum bactericidal concentrations of CLR against cells in a biofilm were higher (1.0 µg/ml) than that for planktonic cells (0.25 µg/ml). It was shown that the expression of efflux pump genes was significantly increased in biofilm cells. In addition, exposure of biofilms to CLR resulted in high level resistance generation compared to planktonic cells with increased resistance associated with the presence of a point mutation at either position 2142 or 2143 in the domain V loop of the 23S rRNA gene. These results demonstrate that H. pylori biofilm formation decreases the susceptibility to CLR and that H. pylori CLR resistance mutations are more frequently generated in biofilms than in planktonic cells.
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影响因子：
9.4
作者：
Kobayashi, Intetsu;Murakami, Kazunari;Fujioka, Toshio
通讯作者：
Fujioka, Toshio
影响因子：
64.8
作者：
Alm, RA;Ling, LSL;Trost, TJ
通讯作者：
Trost, TJ
影响因子：
29.4
作者：
Lind, T;Mégraud, F;Cederberg, C
通讯作者：
Cederberg, C
影响因子：
4.9
作者：
DUNNE, WM;MASON, EO;KAPLAN, SL
通讯作者：
KAPLAN, SL
影响因子：
3.2
作者：
Carron, Michael A.;Tran, Vivian R.;Coticchia, James M.
通讯作者：
Coticchia, James M.