Quantitative proteomics of parotid saliva in primary Sjögren's syndrome.
Quantitative proteomics of parotid saliva in primary Sjögren's syndrome.
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DOI：
10.1002/pmic.201200208
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发表时间：
2012-10
期刊：
影响因子：
3.4
通讯作者：
Melvin, James E.
中科院分区：
文献类型：
--
作者：
Ambatipudi, Kiran S.;Swatkoski, Stephen;Moresco, James J.;Tu, Patricia G.;Coca, Andreea;Anolik, Jennifer H.;Gucek, Marjan;Sanz, Ignacio;Yates, John R., III;Melvin, James E.
原发性干燥综合征（pSS）的诊断是困难的，由于缺乏具体的实验室和临床试验。作为全球发现pSS中腮腺唾液蛋白丰度变化的第一步，通过多维蛋白质鉴定技术（MudPIT）将合并样本与健康对照（HC）受试者的样本进行比较。MudPIT共鉴定出1246个蛋白质。其中477种蛋白质的丰度没有变化，529种仅在pSS或HC样品中检测到，而其中206种蛋白质显著上调≥ 2倍，34种蛋白质下调≤ 0.5倍。通过MudPIT鉴定的差异表达蛋白的免疫途径分析导致鉴定出100个显著途径。使用反相（RP）质谱法平行定量相同的样品。RP鉴定的71个蛋白质中有58个与MudPIT结果重叠。通过靶向无标记定量进一步分析了五种蛋白质，以确认通过RP和MudPIT方法观察到的相似的相对差异表达。本研究支持使用质谱法在全球范围内发现和验证标记蛋白，以改善和早期诊断pSS。
The diagnosis of primary Sjögren’s syndrome (pSS) is difficult due to the lack of specific laboratory and clinical tests. As an initial step for the global discovery of changes in the abundance of parotid salivary proteins in pSS, a pooled sample was compared to that from healthy control (HC) subjects by multidimensional protein identification technology (MudPIT). A total of 1246 proteins were identified by MudPIT. The abundance of 477 of these proteins did not change, 529 were only detected in either the pSS or HC sample, while 206 of these proteins were significantly up-regulated ≥ 2-fold and 34 were down-regulated ≤ 0.5. Ingenuity Pathway Analyses of differentially expressed proteins identified by MudPIT resulted in the identification of 100 significant pathways. The same samples were quantified in parallel using reversed phase (RP) mass spectrometry. Fifty eight of 71 proteins identified by RP overlapped with MudPIT results. Five proteins were further analyzed by targeted label-free quantification to confirm the similar relative differential expression observed by RP and MudPIT approaches. The present study supports the use of mass spectrometry for global discovery and validation of marker proteins for improved and early diagnosis of pSS.
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影响因子：
4.4
作者：
Ambatipudi, Kiran S.;Lu, Bingwen;Hagen, Fred K.;Melvin, James E.;Yates, John R., III
通讯作者：
Yates, John R., III
影响因子：
--
作者：
Peluso, G.;De Santis, M.;Ferraccioli, G. F.
通讯作者：
Ferraccioli, G. F.
影响因子：
4.4
作者：
Peng, JM;Elias, JE;Gygi, SP
通讯作者：
Gygi, SP
影响因子：
4.4
作者：
Tabb, DL;McDonald, WH;Yates, JR
通讯作者：
Yates, JR
影响因子：
7.4
作者：
Liu, HB;Sadygov, RG;Yates, JR
通讯作者：
Yates, JR