Recombinant human sonic hedgehog protein regulates the expression of ZO-1 and occludin by activating angiopoietin-1 in stroke damage.
Recombinant human sonic hedgehog protein regulates the expression of ZO-1 and occludin by activating angiopoietin-1 in stroke damage.
复制标题
重组人声音刺猬蛋白通过在中风损伤中激活Angiopoietin-1来调节ZO-1和occludin的表达。
DOI：
10.1371/journal.pone.0068891
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Xia YP;He QW;Li YN;Chen SC;Huang M;Wang Y;Gao Y;Huang Y;Wang MD;Mao L;Hu B
This study examines the regulating effect of Sonic Hedgehog (Shh) on the permeability of the blood-brain barrier (BBB) in cerebral ischemia. By employing permanent middle cerebral artery occlusion (pMCAO) model, we find that Shh significantly decreases brain edema and preserves BBB permeability. Moreover, Shh increases zonula occludens-1 (ZO-1), occludin and angiopiotetin-1 (Ang-1) expression in the ischemic penumbra. Blockage of Shh with cyclopamine abolishes the effects of Shh on brain edema, BBB permeability and ZO-1, occludin, Ang-1 expression. Primary brain microvessel endothelial cells (BMECs) and astrocytes were pre-treated with Shh, cyclopamine, Ang-1-neutralizing antibody, and subjected to oxygen-glucose deprivation (OGD). Results show that the Ang-1 protein level in the culture medium of Shh-treated astrocytes is significantly higher. Shh also increased ZO-1, occludin and Ang-1 expression in BMECs, while cyclopamine and Ang-1-neutralizing antibody inhibited the effects of Shh on the ZO-1 and occludin expression, respectively. This study suggests that, under ischemic insults, Shh triggers Ang-1 production predominantly in astrocytes, and the secreted Ang-1 acts on BMECs, thereby upregulating ZO-1 and occludin to repair the tight junction and ameliorate the brain edema and BBB leakage.
登录
查看更多内容
影响因子：
4.6
作者：
Gritli-Linde, A;Bei, M;McMahon, AP
通讯作者：
McMahon, AP
影响因子：
5.4
作者：
Lee, Sae-Won;Kim, Woo Jean;Kim, Kyu-Won
通讯作者：
Kim, Kyu-Won
DOI：
10.1016/j.bbrc.2005.11.106
发表时间：
2006-01-20
影响因子：
3.1
作者：
Hashizume, A;Hieda, Y
通讯作者：
Hieda, Y
影响因子：
8.3
作者：
Belayev, L;Alonso, OF;Ginsberg, MD
通讯作者：
Ginsberg, MD
DOI：
10.1007/s11626-009-9270-x
发表时间：
2010-06-01
影响因子：
2.1
作者：
Fujii, Takaaki;Kuwano, Hiroyuki
通讯作者：
Kuwano, Hiroyuki