Conjunctival reconstruction via enrichment of human conjunctival epithelial stem cells by p75 through the NGF-p75-SALL2 signaling axis.
Conjunctival reconstruction via enrichment of human conjunctival epithelial stem cells by p75 through the NGF-p75-SALL2 signaling axis.
复制标题
通过 NGF-p75-SALL2 信号轴通过 p75 富集人结膜上皮干细胞进行结膜重建
DOI：
10.1002/sctm.19-0449
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发表时间：
2020-11
影响因子：
6
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Wu N;Yan C;Chen J;Yao Q;Lu Y;Yu F;Sun H;Fu Y
严重的结膜疾病会导致明显的结膜疤痕，严重限制眼球运动并影响患者的视力。由于缺乏有效的干细胞富集方法，结膜重建仍然具有挑战性。本研究表明，p75阳性结膜上皮细胞（CjECs）主要位于人结膜上皮的基底层，在体内表现出未成熟的分化状态。通过免疫磁珠富集的p75强阳性（p75++）CjEC表现出干细胞标志物的高表达和分化角蛋白的低表达。在连续细胞传代培养过程中，p75++ CjECs表现出最强的增殖潜力，能够在体内重建结构和功能最完整的结膜。外源添加 NGF 通过增加 p75++ CjEC 中 SALL2 的核定位来促进 CjEC 的分化，而 proNGF 则发挥相反的作用。综上所述，p75++ CjECs具有干细胞特征，具有最强的增殖潜力，可作为结膜重建的种子细胞，且NGF-p75-SALL2信号通路参与调节CjECs的分化。采用免疫磁珠富集p75++ CjECs（结膜上皮细胞）并检测其干细胞特性。羊膜上培养10天后，将p75++/− CjECs构建的结膜移植到兔结膜缺损模型中，测试修复效果。外源添加 NGF 通过增加 p75++ CjEC 中 SALL2 的核定位来促进 CjEC 的分化。
Severe conjunctival diseases can cause significant conjunctival scarring, which seriously limits eye movement and affects patients' vision. Conjunctival reconstruction remains challenging due to the lack of efficient methods for stem cells enrichment. This study indicated that p75 positive conjunctival epithelial cells (CjECs) were mainly located in the basal layer of human conjunctival epithelium and showed an immature differentiation state in vivo. The p75 strongly positive (p75++) CjECs enriched by immuno‐magnetic beads exhibited high expression of stem cell markers and low expression of differentiated keratins. During continuous cell passage cultivation, p75++ CjECs showed the strongest proliferation potential and were able to reconstruct the conjunctiva in vivo with the most complete structure and function. Exogenous addition of NGF promoted the differentiation of CjECs by increasing nuclear localization of SALL2 in p75++ CjECs while proNGF played an opposite role. Altogether, p75++ CjECs present stem cell characteristics and exhibit the strongest proliferation potential so can be used as seed cells for conjunctival reconstruction, and NGF‐p75‐SALL2 signaling pathway was involved in regulating the differentiation of CjECs. p75++ CjECs(conjunctival epithelial cells) were enriched by immuno‐magnetic beads and their stem cell properties were detected. After 10 days of culture on amniotic membrane, conjunctiva constructed by p75++/− CjECs was transplanted to a rabbit model of conjunctival defect to test the repair effect. Exogenous addition of NGF promoted the differentiation of CjECs by increasing nuclear localization of SALL2 in p75++ CjECs.
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影响因子：
4.4
作者：
Lambiase, Alessandro;Micera, Alessandra;Bonini, Stefano
通讯作者：
Bonini, Stefano
同种异体培养式角膜缘上皮移植治疗严重眼部烧伤引起的睑缘联合的远期疗效
影响因子：
2
作者：
Cheng J;Zhai H;Wang J;Duan H;Zhou Q
通讯作者：
Zhou Q
DOI：
10.1016/j.jtos.2012.07.002
发表时间：
2013-01
期刊：
The ocular surface
影响因子：
--
作者：
Kao WW;Liu H;Zhang J
通讯作者：
Zhang J
影响因子：
2.4
作者：
Ishii A;Muramatsu T;Lee JM;Higa K;Shinozaki N;Jung HS;Shibahara T
通讯作者：
Shibahara T
DOI：
10.1073/pnas.82.16.5390
发表时间：
1985-01-01
影响因子：
11.1
作者：
BARRANDON, Y;GREEN, H
通讯作者：
GREEN, H