Expression of p75(NGFR), a Proliferative and Basal Cell Marker, in the Buccal Mucosa Epithelium during Re-epithelialization.
Expression of p75(NGFR), a Proliferative and Basal Cell Marker, in the Buccal Mucosa Epithelium during Re-epithelialization.
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DOI：
10.1267/ahc.14011
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发表时间：
2014-08-29
影响因子：
2.4
通讯作者：
Shibahara T
中科院分区：
文献类型：
--
作者：
Ishii A;Muramatsu T;Lee JM;Higa K;Shinozaki N;Jung HS;Shibahara T
为了阐明上皮干细胞在创面愈合过程中的作用，我们研究了p75NGFR在创面愈合过程中增殖和基础细胞标志物p75NGFR在小鼠颊黏膜上皮的表达。用CO2激光照射6周龄小鼠颊黏膜上皮，造成口腔黏膜上皮损伤。小鼠在激光照射后立即给予BrdU。然后在1、3、7和14天后处死动物。制备石蜡切片，用抗p75NGFR、BrdU、增殖细胞核抗原和CK14抗体进行免疫组织化学染色。在再上皮化过程中，增殖细胞核抗原(-)/p75NGFR(+)细胞延伸至创面，然后闭合，而在创口边缘未见增殖细胞核抗原(+)/p75NGFR(+)细胞。此外，p75NGFR(-)/CK14(+)表达在扩张上皮基底层，反映了有丝分裂后分化细胞的存在。BrdU(+)/p75NGFR(+)反映上皮干细胞的存在，愈合后在颊基底层细胞中可见少量表达，7d后消失。这些结果表明，p75NGFR(+)角质形成细胞定位于口腔黏膜上皮干细胞所在的基底层，并保持增殖能力以再生口腔黏膜上皮。
We investigated the expression of p75NGFR, a proliferative and basal cell marker, in the mouse buccal mucosa epithelium during wound healing in order to elucidate the role of epithelial stem cells. Epithelial defects were generated in the epithelium of the buccal mucosa of 6-week-old mice using CO2 laser irradiation. BrdU was immediately administered to mice following laser irradiation. They were then sacrificed after 1, 3, 7, and 14 days. Paraffin sections were prepared and the irradiated areas were analyzed using immunohistochemistry with anti-p75NGFR, BrdU, PCNA, and CK14 antibodies. During re-epithelialization, PCNA (–)/p75NGFR (+) cells extended to the wound, which then closed, whereas PCNA (+)/p75NGFR (+) cells were not observed at the edge of the wound. In addition, p75NGFR (–)/CK14 (+), which reflected the presence of post-mitotic differentiating cells, was observed in the supra-basal layers of the extended epithelium. BrdU (+)/p75NGFR (+), which reflected the presence of epithelial stem cells, was detected sparsely in buccal basal epithelial cells after healing, and disappeared after 7 days. These results suggest that p75NGFR (+) keratinocytes are localized in the basal layer, which contains oral epithelial stem cells, and retain the ability to proliferate in order to regenerate the buccal mucosal epithelium.
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影响因子：
46.9
作者：
Morris, RJ;Liu, YP;Cotsarelis, G
通讯作者：
Cotsarelis, G
影响因子：
64.8
作者：
Mills, AA;Zheng, BH;Bradley, A
通讯作者：
Bradley, A
影响因子：
64.8
作者：
Yue, ZC;Jiang, TX;Chuong, CM
通讯作者：
Chuong, CM
DOI：
10.1073/pnas.1734203100
发表时间：
2003-09-30
影响因子：
11.1
作者：
Alonso, L;Fuchs, E
通讯作者：
Fuchs, E
DOI：
10.1073/pnas.061032098
发表时间：
2001-03-13
影响因子：
11.1
作者：
Pellegrini, G;Dellambra, E;De Luca, M
通讯作者：
De Luca, M