Molecular Characterization by Multilocus Sequence Typing and Diversity Analysis of Rickettsia asembonensis in Peru.
Molecular Characterization by Multilocus Sequence Typing and Diversity Analysis of Rickettsia asembonensis in Peru.
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通过多头骨序列分型和秘鲁人力苯甲状腺的多样性分析的分子表征。
DOI：
10.1089/vbz.2021.0077
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Leguia M
中科院分区：
文献类型：
--
作者：
Loyola S;Torre A;Flores-Mendoza C;Kocher C;Salmon-Mulanovich G;Richards AL;Leguia M
尽管世界各地有几份报告记录了从体外寄生虫、动物和最近的人类提取的样本中存在阿森立克次体，但这些标本的基因组信息仍然很少，即使有，通常也仅限于价值有限的小基因组片段。我们从秘鲁猫蚤和狗蚤中检测到的五个Asembonens DNA样本中，获得了两个保守基因(17-kDa抗原基因和GLTA)和三个可变区基因(sca4、ompB和OmpA)的完整序列。利用完整的基因序列进行多位点序列分型和系统发育分析，以评估多样性和推断菌株与其他参考序列之间的关系。17 kDa抗原基因在立克次体中高度保守。在可变基因中，ompB的变异性最大，但这种多样性并不是仅通过系统发育学就能捕捉到的，即使在努力使跳蚤物种、动物宿主和位置方面的潜在多样性最大化的情况下也是如此。通过从头开始和基于参考的基因组组装的组合，我们在OmpA中发现了一个75 的插入片段，它编码了在其他立克次体物种中发现的25个氨基酸重复基序，但在肯尼亚的原始原型菌株中不存在。直到最近才被证明是一种真正的人类病原体。因此，再加上缺乏可用的基因组信息，它的研究仍然不足。我们的工作直接解决了世界范围内缺乏可用于研究这些新立克次体物种的基因组信息的问题，并特别有助于我们了解秘鲁阿森邦氏立克次体的多样性和分子流行病学。
Despite several reports worldwide documenting the presence of Rickettsia asembonensis in samples derived from ectoparasites, animals and more recently humans, genomic information of these specimens remains scarce, and when available, is usually limited to small genomic fragments of limited value. We generated complete sequences for two conserved (17-kDa antigen gene and gltA) and three variable (sca4, ompB and ompA) genes in five R. asembonensis DNA samples detected in cat and dog fleas in Peru. Complete gene sequences were used to conduct multi-locus sequence typing and phylogenetic analyses to assess diversity and infer relationships among strains and other reference sequences. The 17-kDa antigen gene was highly conserved across Rickettsia species. Of the variable genes ompB was the most variable, but this diversity was not captured through phylogenetics alone even when efforts were made to maximize potential diversity in terms of flea species, animal host and location. Through a combination of de novo and reference-based genome assembly we identified a 75 bp insertion in ompA that encodes a 25 aa repetitive motif found in other Rickettsia species, but not present in the original prototype strain from Kenya. R. asembonensis has only recently been shown to be a bona-fide human pathogen. As such, and compounded by a lack of available genomic information, it remains understudied. Our work directly addresses the lack of genomic information available worldwide for the study of these novel Rickettsia species and specifically contributes to our understanding of the diversity and molecular epidemiology of R. asembonensis in Peru.
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影响因子：
9.3
作者：
Beck TF;Mullikin JC;NISC Comparative Sequencing Program;Biesecker LG
通讯作者：
Biesecker LG
影响因子：
4.6
作者：
Arteche-López A;Ávila-Fernández A;Romero R;Riveiro-Álvarez R;López-Martínez MA;Giménez-Pardo A;Vélez-Monsalve C;Gallego-Merlo J;García-Vara I;Almoguera B;Bustamante-Aragonés A;Blanco-Kelly F;Tahsin-Swafiri S;Rodríguez-Pinilla E;Minguez P;Lorda I;Trujillo-Tiebas MJ;Ayuso C
通讯作者：
Ayuso C
影响因子：
2.1
作者：
Blanton, Lucas S.;Vohra, Rahat F.;Bouyer, Donald H.
通讯作者：
Bouyer, Donald H.
影响因子：
2.1
作者：
Faccini-Martinez, Alvaro A.;Ramirez-Hernandez, Alejandro;Hidalgo, Marylin
通讯作者：
Hidalgo, Marylin
影响因子：
2.1
作者：
Flores-Mendoza, Carmen;Florin, David;Richards, Allen L.
通讯作者：
Richards, Allen L.