Binding of plasma proteins to Candida species in vitro.
Binding of plasma proteins to Candida species in vitro.
复制标题
体外血浆蛋白与念珠菌属物种的结合。
DOI：
--
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
F. C. Odds
中科院分区：
文献类型：
--
作者：
S. Page;F. C. Odds
纯化的人白蛋白，纤维蛋白原和转铁蛋白结合念珠菌属的能力，通过免疫荧光法测定。这些蛋白质都能与白色念珠菌形成的芽管结合，但不与念珠菌芽生孢子结合。白色念珠菌或其他致病性念珠菌属，甚至不能与带有芽管的母芽生孢子结合。蛋白质与C的结合程度。白色念珠菌芽管在生长培养基之间和芽管与芽管之间变化。梭菌菌株不形成芽管的白色念珠菌不能结合任何蛋白质。有证据表明，纯化的纤维蛋白原结合到胚芽管具有更高的亲合力比白蛋白和转铁蛋白。当用全人血浆或血清处理生殖管时，间接免疫荧光显示蛋白质结合在C.白色念珠菌芽生孢子-芽管单位，表明行为不同于单独或混合测试的纯化蛋白质。C.在唑类抗真菌剂存在下生长的白色念珠菌细胞以与未用药物处理的细胞相同的方式结合纯化的血浆蛋白。本研究结果表明，宿主蛋白与C.白色念珠菌可能不是与真菌在体内的毒力直接相关的性质。
The ability of purified human albumin, fibrinogen and transferrin to bind to Candida species was measured by immunofluorescence. The proteins all bound with high avidity to germ-tubes formed by Candida albicans, but did not bind to blastospores of C. albicans or other pathogenic Candida species, not even to parent blastospores bearing germ-tubes. The extent of binding of the proteins to C. albicans germ-tubes varied between growth media and from germ-tube to germ-tube. Strains of C. albicans that did not form germ-tubes were incapable of binding any of the proteins. There was evidence that purified fibrinogen bound to germ-tubes with higher avidity than albumin and transferrin. When germ-tubes were treated with whole human plasma or serum, indirect immunofluorescence revealed that proteins were bound all over the surface of C. albicans blastospore-germ-tube units, indicating behaviour different from that seen with the purified proteins tested alone or in mixtures. C. albicans cells grown in the presence of azole antifungal agents bound purified plasma proteins in the same way as cells untreated with the drugs. The results of this study suggest that binding of host proteins to the surface of C. albicans may not be a property related directly to virulence of the fungus in vivo.
影响因子：
4.4
作者：
J. Edwards;T. Gaither;J. O’Shea;D. Rotrosen;T. Lawley;S. Wright;M. Frank;I. Green
通讯作者：
J. Edwards;T. Gaither;J. O’Shea;D. Rotrosen;T. Lawley;S. Wright;M. Frank;I. Green
念珠菌物种对宿主组织和塑料表面的粘附。
DOI：
10.1093/clinids/8.1.73
发表时间：
1986
期刊：
Reviews of infectious diseases
影响因子：
--
作者：
Rotrosen,D;Calderone,RA;EdwardsJr,JE
通讯作者：
EdwardsJr,JE