A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of P. falciparum and analysis of nucleic acids.
A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of P. falciparum and analysis of nucleic acids.
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DOI：
10.1080/01478885.2022.2088191
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发表时间：
2022-09
影响因子：
1.1
通讯作者：
中科院分区：
文献类型：
--
作者：
在低资源环境中收集、保存和运输组织学标本具有挑战性。我们提出了一种新的方法，实现了良好的保存胎盘标本从农村马里通过使用福尔马林固定，乙醇脱水，并在太阳能冰箱长期储存。样本保存成功率为92%，允许评估当前和过去的疟疾感染，贫血，胎盘成熟度和炎症。使用RNAscope®杂交，我们能够可视化福尔马林固定石蜡包埋（FFPE）标本中的细胞特异性基因表达模式。此外，我们的方法需要从子叶的两个切割面进行镜像采样，一个用于FFPE工作流程，另一个用于存储在RNAlater™和RNA-seq中。
Collection, preservation, and shipment of histological specimens in low-resource settings is challenging. We present a novel method that achieved excellent preservation of placental specimens from rural Mali by using formalin fixation, ethanol dehydration, and long-term storage in a solar-powered freezer. Sample preservation success was 92%, permitting evaluation of current and past malaria infection, anemia, placental maturity, and inflammation. Using RNAscope® hybridization we were able to visualize cell-specific gene expression patterns in the formalin-fixed paraffin-embedded (FFPE) specimens. Additionally, our method entailed mirrored sampling from the two cut faces of a cotyledon, one for the FFPE workflows and the other for storage in RNAlater™ and RNA-seq.
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影响因子：
1.7
作者：
Velez, Ana Maria Abreu;Howard, Michael S.;Smoller, Bruce
通讯作者：
Smoller, Bruce
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Teichmann SA
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Gomez-Lopez, Nardhy
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通讯作者：
Luo, Yuling
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