Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker.
Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker.
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分子 NIR-II 细胞追踪仪揭示移植干细胞对患病环境的差异反应
DOI：
10.34133/2021/9798580
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Chen H;Yang H;Zhang C;Chen S;Zhao X;Zhu M;Wang Z;Wang Y;Wo HT;Li K;Cheng Z
干细胞疗法在再生医学中有着很高的前景。临床翻译的主要挑战是精确和定量地评估体内细胞的分布、迁移和植入，这是目前技术无法轻易实现的。为了解决这个问题，我们首次开发了一种分子细胞跟踪器，在第二个近红外（NIR-II）窗口（1,000-1,700 nm）具有强荧光信号，用于实时监测健康和患病动物模型的体内细胞行为。NIR-II追踪器（CelTrac1000）显示出完整的细胞标记，具有低细胞毒性和30天高时空分辨率的长期跟踪能力，可用于移植干细胞生物分布的半量化。利用CelTrac1000的独特优点，移植干细胞对不同病变环境的反应被区分和揭示。此外，我们还证明了CelTrac1000是一种通用且有效的技术，可以在100 μm的单细胞簇空间分辨率下超快速实时跟踪细胞迁移和分布，以及肺收缩和心脏跳动。因此，这种NIR-II跟踪器将把光学细胞跟踪转变为单细胞簇和毫秒时间分辨率，以便更好地评估和了解干细胞治疗，提供最佳剂量和疗效。
Stem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1,000-1,700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100 μm single-cell cluster spatial resolution, along with the lung contraction and heart beating. As such, this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy.
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影响因子：
46.9
作者：
Lane, Steven W.;Williams, David A.;Watt, Fiona M.
通讯作者：
Watt, Fiona M.
DOI：
10.1126/science.1247391
发表时间：
2014-08-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fox IJ;Daley GQ;Goldman SA;Huard J;Kamp TJ;Trucco M
通讯作者：
Trucco M
影响因子：
16.6
作者：
Antaris AL;Chen H;Diao S;Ma Z;Zhang Z;Zhu S;Wang J;Lozano AX;Fan Q;Chew L;Zhu M;Cheng K;Hong X;Dai H;Cheng Z
通讯作者：
Cheng Z
影响因子：
41.2
作者：
Huang, Jiaguo;Li, Jingchao;Pu, Kanyi
通讯作者：
Pu, Kanyi
影响因子：
9.5
作者：
Chetty, Shashank Shankar;Praneetha, Selvarasu;Murugan, Arumugam Vadivel
通讯作者：
Murugan, Arumugam Vadivel