Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system.
Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system.
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DOI：
10.1038/srep05089
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发表时间：
2014-05-28
影响因子：
4.6
通讯作者：
Nakazawa T
中科院分区：
文献类型：
--
作者：
Tanaka Y;Tsuda S;Kunikata H;Sato J;Kokubun T;Yasuda M;Nishiguchi KM;Inada T;Nakazawa T
房水是眼内的一种体液，与多种眼部疾病有关，但目前尚不清楚房水在患者体内的完整RNA特征。这项研究的目的是，通过微阵列分析，揭示单个AH患者与疾病相关的细胞外miRNAs谱。前房穿刺术收集10例青光眼、5例白内障和5例视网膜前膜患者的 μ。提取的总RNA小于2 0 0 nT，平均含量为5.2 7±0.4 1 ng。在每个样本中检测到530.5±44.6miRNA类型的2019年可检测的成熟miRNAs中，在所有10名青光眼患者或对照组中均检测到172miRNAs。在青光眼组中，11个显著上调的miRNAs和18个显著下调的miRNAs(两者均为P<；0.05)在受试者操作特征分析中发现曲线下面积大于0.74。他们还在分级聚类分析中形成了一个仅由青光眼患者组成的集群。AH有可能成为miRNA的来源，作为生物标记物和治疗靶点。
Aqueous humor (AH) is one of the body fluids in the eye, which is known to be related with various ocular diseases, but the complete RNAs characteristic of the AH in patients is not yet known. The aim of this study was, with a microarray analysis, to reveal the disease-related extracellular miRNAs profiles in individual patients AH. 100 μl of AH was collected by anterior chamber paracentesis from 10 glaucoma, 5 cataract, and 5 epiretinal membrane patients. The extracted total RNAs were shorter than 200 nt, and their amount was 5.27 ± 0.41 ng in average. Among 530.5 ± 44.6 miRNA types detected in each sample with a microarray detectable 2019 types of matured miRNAs, 172 miRNAs were detected in all 10 glaucoma or control patients. From the glaucoma group, 11 significantly up-regulated and 18 significantly down-regulated miRNAs (P < 0.05 for both) were found to have areas under the curve better than 0.74 in a receiver operating characteristic analysis. They also formed a cluster composed only of glaucoma patients in a hierarchal cluster analysis. AH had a possibility of becoming a source of miRNA that can serve as a biomarker and a therapeutic target.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
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作者：
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通讯作者：
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DOI：
10.1186/1758-907x-1-7
发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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