Exploring the human cerebral cortex using confocal microscopy
Exploring the human cerebral cortex using confocal microscopy
复制标题
使用共聚焦显微镜探索人类大脑皮层
DOI：
10.1101/2021.07.16.452651
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Luca Pesce, Annunziatina Laurino
中科院分区：
文献类型：
--
作者：
Luca Pesce, Annunziatina Laurino
对人脑中神经元分布的全覆盖映射将对深入了解大脑功能产生重大影响。因此，在细胞水平上完全了解人类大脑不同区域的结构组织将有助于理解它们在特定神经网络功能中的作用。组织清除技术的最新进展使这一目标取得了重要进展。这些方法使用能够溶解脂质的特定化学物质，通过均化折射率使组织完全透明。然而，标记和清除人脑样本仍然具有挑战性。在这里，我们提出了一种结合免疫染色、Switch/TDE透明和共聚焦显微镜的方法来执行人类大脑皮层的细胞图谱。提供了对由组织固定产生的自体荧光信号的贡献的具体评估以及对脂褐素色素干扰的评估。我们的评估表明，可以获得一个有效的清除和标记成人脑片部分的过程，使其成为神经元和非神经元标记物的形态分类和抗体验证的极佳方法。
Cover-all mapping of the distribution of neurons in the human brain would have a significant impact on the deep understanding of brain function. Therefore, complete knowledge of the structural organization of different human brain regions at the cellular level would allow understanding their role in the functions of specific neural networks. Recent advances in tissue clearing techniques have allowed important advances towards this goal. These methods use specific chemicals capable of dissolving lipids, making the tissue completely transparent by homogenizing the refractive index. However, labeling and clearing human brain samples is still challenging. Here, we present an approach to perform the cellular mapping of the human cerebral cortex coupling immunostaining with SWITCH/TDE clearing and confocal microscopy. A specific evaluation of the contributions of the autofluorescence signals generated from the tissue fixation is provided as well as an assessment of lipofuscin pigments interference. Our evaluation demonstrates the possibility of obtaining an efficient clearing and labeling process of parts of adult human brain slices, making it an excellent method for morphological classification and antibody validation of neuronal and non-neuronal markers.
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DOI：
10.1023/a:1017925023408
发表时间：
2001-05-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
Elleder, M;Borovansky, J
通讯作者：
Borovansky, J
影响因子：
2
作者：
Oliveira, V. C.;Carrara, R. C. V.;Neder, L.
通讯作者：
Neder, L.
影响因子：
3.4
作者：
Costantini, Irene;Mazzamuto, Giacomo;Pavone, Francesco Saverio
通讯作者：
Pavone, Francesco Saverio
影响因子：
1.1
作者：
Sarnat HB
通讯作者：
Sarnat HB