Anopheles gambiae complex along The Gambia river, with particular reference to the molecular forms of An. gambiae s.s.
Anopheles gambiae complex along The Gambia river, with particular reference to the molecular forms of An. gambiae s.s.
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DOI：
10.1186/1475-2875-7-182
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发表时间：
2008-09-22
期刊：
影响因子：
3
通讯作者：
della Torre A
中科院分区：
文献类型：
--
作者：
Caputo B;Nwakanma D;Jawara M;Adiamoh M;Dia I;Konate L;Petrarca V;Conway DJ;della Torre A
主要非洲热带疟疾媒介种冈比亚按蚊M和S分子型的地理和时间分布。在其分布范围的西端，从未被详细研究过。收藏室内休息安。冈比亚公司雌性沿着ca.在2005年雨季结束/旱季初期和2006年雨季期间，沿冈比亚河从冈比亚西部沿海地区到塞内加尔东南部的400公里西向东样带。采用PCR-RFLP技术对样本进行种属鉴定和分子形式鉴定，并通过ELISA试验确定进食雌虫的血粉来源。四千多安。冈比亚有限公司2005年和2006年分别收集和鉴定了1 041名和3 038名成年女性。在样带的西部，M型主要与黑按蚊和S型同域分布，中部主要与阿拉伯按蚊同域分布。S型在塞内加尔东部的苏丹-几内亚萨凡纳地区流行，与An.阿拉伯的。黑按蚊（Anopheles melas）和黑按蚊（An.在雨季样品中，阿拉伯按蚊的相对频率普遍较低，而在雨季样品中，阿拉伯按蚊的相对频率较低。冈比亚公司占了上风没有观察到大的季节性波动的M和S型。在既记录了M又记录了S的地区，它们之间的杂交频率为0.6%至7%。观察到的模式分类群的分布支持的假设，一个更好的适应M-形式的特点是保持水的冲积矿床沿着冈比亚河，沼泽植被，红树林和水稻种植的特点。相比之下，S型似乎更好地适应自由排水的土壤，覆盖着开阔的林地萨凡纳或农田，丰富的临时幼虫繁殖地，主要特征是东部的样带，冈比亚河的环境影响要小得多深刻和农业活动主要依赖于雨水。非常有趣的是，观察到的分子形式之间的杂交频率沿着整个样带是远远高于迄今为止已报道的其他地区。研究结果支持了M型和S型之间的生物学差异，并表明An.冈比亚公司地理分布可以代表两种分子形式之间杂交高于预期的区域。
The geographic and temporal distribution of M and S molecular forms of the major Afrotropical malaria vector species Anopheles gambiae s.s. at the western extreme of their range of distribution has never been investigated in detail. Collections of indoor-resting An. gambiae s.l. females were carried out along a ca. 400 km west to east transect following the River Gambia from the western coastal region of The Gambia to south-eastern Senegal during 2005 end of rainy season/early dry season and the 2006 rainy season. Specimens were identified to species and molecular forms by PCR-RFLP and the origin of blood-meal of fed females was determined by ELISA test. Over 4,000 An. gambiae s.l. adult females were collected and identified, 1,041 and 3,038 in 2005 and 2006, respectively. M-form was mainly found in sympatry with Anopheles melas and S-form in the western part of the transect, and with Anopheles arabiensis in the central part. S-form was found to prevail in rural Sudan-Guinean savannah areas of Eastern Senegal, in sympatry with An. arabiensis. Anopheles melas and An. arabiensis relative frequencies were generally lower in the rainy season samples, when An. gambiae s.s. was prevailing. No large seasonal fluctuations were observed for M and S-forms. In areas where both M and S were recorded, the frequency of hybrids between them ranged from to 0.6% to 7%. The observed pattern of taxa distribution supports the hypothesis of a better adaptation of M-form to areas characterized by water-retaining alluvial deposits along the Gambia River, characterized by marshy vegetation, mangrove woods and rice cultivations. In contrast, the S-form seems to be better adapted to free-draining soil, covered with open woodland savannah or farmland, rich in temporary larval breeding sites characterizing mainly the eastern part of the transect, where the environmental impact of the Gambia River is much less profound and agricultural activities are mainly rain-dependent. Very interestingly, the observed frequency of hybridization between the molecular forms along the whole transect was much higher than has been reported so far for other areas. The results support a bionomic divergence between the M and S-forms, and suggest that the western extreme of An. gambiae s.s. geographical distribution may represent an area of higher-than-expected hybridization between the two molecular forms.
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DOI：
10.4269/ajtmh.2008.78.169
发表时间：
2008-01-01
影响因子：
3.3
作者：
Calzetta, Maria;Santolamazza, Federica;Petrarca, Vincenzo
通讯作者：
Petrarca, Vincenzo
疟疾媒介在农村小镇和冈比亚卫星村庄的居住风险因素。
影响因子：
3
作者：
Kirby, Matthew J.;Green, Clare;Milligan, Paul M.;Sismanidis, Charalambos;Jasseh, Momadou;Conway, David J.;Lindsay, Steven W.
通讯作者：
Lindsay, Steven W.
影响因子：
1.5
作者：
BRYAN, JH;DIDECO, MA;COLUZZI, M
通讯作者：
COLUZZI, M
影响因子：
2.1
作者：
Bogh, C;Clarke, SE;Lindsay, SW
通讯作者：
Lindsay, SW
影响因子：
2.1
作者：
Lemasson, JJ;Fontenille, D;Molez, JF
通讯作者：
Molez, JF