Optimizing electrospray interfaces using slowly diverging conical duct (ConDuct) electrodes.
Optimizing electrospray interfaces using slowly diverging conical duct (ConDuct) electrodes.
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DOI：
10.1007/s13361-014-1063-0
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发表时间：
2015-04
影响因子：
3.2
通讯作者：
Chait BT
中科院分区：
文献类型：
--
作者：
Krutchinsky AN;Padovan JC;Cohen H;Chait BT
我们证明，离子传输的效率从大气到真空通过不锈钢电极，包含缓慢发散的锥形管道（Conduct）通道可以接近100%。在这里，我们探索了2.5 cm长电极的特性，发散角分别为0°、1°、2°、3°、5°、8°、13°和21°。观察到离子传输效率从0°（直）通道的10-20%跃升至发散角小至1°的通道的90-95%。此外，2-3° Conduct电极产生了非常低的发散离子束，这些离子束在真空中以类似激光的方式长距离传播。为了利用这些新发现的特性，我们构建了一种新型的大气-真空离子界面，利用2° Conduct作为入口电极，并将其离子传输效率与商业（Thermo）Velos Orbitrap和Q Exactive质谱仪中使用的界面进行了比较。我们观察到，ConDuct接口传输的离子比商业参考接口多17倍，并且还产生了改进的肽的信噪比质谱。我们从这些结果中推断，许多目前的大气压到真空接口使用金属毛细管的性能可以通过将它们替换为1°或2°金属导管电极来显著改善，这应该保留通过加热电极来提供离子去溶剂化能量的便利性，同时大大提高离子传输到质谱仪中的效率。
We demonstrate that the efficiency of ion transmission from atmosphere to vacuum through stainless steel electrodes that contain slowly divergent conical duct (ConDuct) channels can be close to 100%. Here, we explore the properties of 2.5 cm long electrodes with angles of divergence of 0°, 1°, 2°, 3°, 5°, 8°, 13°, and 21°, respectively. The ion transmission efficiency was observed to jump from 10–20% for the 0° (straight) channels to 90–95% for channels with an angle of divergence as small as 1°. Furthermore, the 2–3° ConDuct electrodes produced extraordinarily low divergence ion beams that propagated in a laser-like fashion over long distances in vacuum. To take advantage of these newly discovered properties, we constructed a novel atmosphere-to-vacuum ion interface utilizing a 2° ConDuct as an inlet electrode and compared its ion transmission efficiency with that of the interface used in the commercial (Thermo) Velos Orbitrap and Q Exactive mass spectrometers. We observed that the ConDuct interface transmitted up to 17 times more ions than the commercial reference interface and also yielded improved signal-to-noise mass spectra of peptides. We infer from these results that the performance of many current atmosphere-tovacuum interfaces utilizing metal capillaries can be substantially improved by replacing them with 1° or 2° metal ConDuct electrodes, which should preserve the convenience of supplying ion desolvation energy by heating the electrode while greatly increasing the efficiency of ion transmission into the mass spectrometer.
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DOI：
10.1016/j.jasms.2007.05.018
发表时间：
2007-09-01
影响因子：
3.2
作者：
Page, Jason S.;Kelly, Ryan T.;Smith, Richard D.
通讯作者：
Smith, Richard D.
影响因子：
5.2
作者：
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通讯作者：
McMurry, PH
影响因子：
5.2
作者：
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通讯作者：
McMurry, PH
影响因子：
1.8
作者：
WILM, MS;MANN, M
通讯作者：
MANN, M
影响因子：
1.6
作者：
KANTROWITZ, A;GREY, J
通讯作者：
GREY, J