Electron microscopic evidence on the acrosomal status of bound sperm and their penetration into human hemizonae pellucida after storage in a buffered salt solution *
Electron microscopic evidence on the acrosomal status of bound sperm and their penetration into human hemizonae pellucida after storage in a buffered salt solution *
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关于结合精子顶体状态及其在缓冲盐溶液中储存后渗透到人透明半带中的电子显微镜证据*
作者：
Daniel R. Franken;W. T. Oosthuizen;S. Cooper;T. Kruger;L. J. Burkman;Charles C. Coddington;G. D. Hodgen
摘要半透明带试验（HZA）是一种用显微切割的人透明带评价精子结合能力的方法。在这项研究中，8个人卵母细胞在缓冲盐溶液中储存60天，分为两个相同的半球（hemizonae）和共孵育的精子从一个有生育力的男子。所有评估的精子紧密结合到外表面或已开始渗透到透明质膜。使用标准技术制备并固定具有结合精子的半带用于透射电子显微镜（TEM）。在108个结合到该区域的精子中，我们能够通过TEM评估25个。20个（80%）与精子结合的精子是部分或完全顶体反应，而6个（20%）与精子结合的精子具有完整的顶体。精子顶体完整、部分顶体反应和完全反应的精子均存在于顶体中。在骨基质内观察到穿透路径或隧道。结果表明，精子与透明膜紧密结合时，通常会诱导顶体反应。重要的是，在盐溶液（缓冲至7.4）中储存人卵后，透明卵保持其生物学和功能特性至少90天。
Summary. The hemizona assay (HZA) was developed to evaluate sperm binding potential using microbisected human zona pellucida. In this study, eight human oocytes stored in a buffered salt solution for 60 days were bisected into two identical hemispheres (hemizonae) and coincubated with the spermatozoa from a fertile man. All evaluated spermatozoa were tightly bound to the outer surface or had begun penetration into the zona pellucida. The hemizonae with bound spermatozoa were prepared and fixed for transmission electron microscopy (TEM) using standard techniques. Among the 108 sperm bound to the zone we were able to evaluate 25 by TEM. Twenty (80%) of the zona bound spermatozoa were partially or completely acrosome reacted, while six (20%) of the zona bound sperm had intact acrosomes. Acrosome intact, partially acrosome reacted and completely reacted spermatozoa were observed within the zona. Penetration pathways or tunnels were seen within the zona matrix. The results illustrate, that typically spermatozoa tightly bound the human zona pellucida show induction of the acrosome reaction. Importantly, following storage of human eggs in salt solution (buffered to 7.4), the zona pellucida retain their biological and functional characteristics for at least 90 days.
盐储存仓鼠和人卵的透明带：同源和异源精子的渗透性。
DOI：
10.1002/mrd.1120210202
发表时间：
1988
期刊：
Gamete research
影响因子：
--
作者：
Yoshimatsu,N;Yanagimachi,R;Lopata,A
通讯作者：
Lopata,A
影响因子：
3.6
作者：
CROSS, NL;MORALES, P;HANSON, FW
通讯作者：
HANSON, FW