HTT2 promotes plant thermotolerance in Brassica rapa.
HTT2 promotes plant thermotolerance in Brassica rapa.
复制标题
HTT2 促进油菜的耐热性
DOI：
10.1186/s12870-018-1346-x
复制
发表时间：
2018-06-20
影响因子：
5.3
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Jiang J;Bai J;Li S;Li X;Yang L;He Y
背景：许多调控基因参与植物耐热性。在拟南芥中，热诱导的TAS 1靶基因2（HTT 2）是一个重要的耐热基因，其被反式作用siRNA ta-siR 255沉默。ta-siR 255不存在于大白菜（Brassica rapa ssp. pekinensis）。本研究以大白菜（B.拉帕湾pekinensis）由于共抑制而失败。理论上，在ta-siR 255介导的基因沉默缺失和共表达潜力较弱的情况下，大白菜可以过量表达拟南芥HTT 2来提高耐热性。我们测试了经受高温（38 °C）和热激（46 °C）处理的p35 S：：HTT 2植物的叶电导率、下胚轴伸长和存活百分比。p35 S：：HTT 2幼苗叶片电导率在高温下下降，但在热激下变化不大。高温和热激处理使p35 S：：HTT 2幼苗下胚轴长度增加。p35 S：：HTT 2幼苗在热激下存活率增加。在高温和热激条件下，p35 S：：HTT 2植物中热激因子基因的一个子集BrpHsfs表达上调。在田间试验中，转HTT 2基因植株比野生型植株更绿，叶球形成时间更早。结论：外源HTT 2通过降低电导率和延长下胚轴长度来提高热激结球白菜的耐热性，从而提高了结球白菜的存活率。本研究为通过外源耐热基因的导入进行作物耐热性的遗传操作提供了一条新途径。
Background:Numerous regulatory genes participate in plant thermotolerance. In Arabidopsis, HEAT-INDUCED TAS1 TARGET2 (HTT2) is an important thermotolerance gene that is silenced by ta-siR255, a trans-acting siRNA. ta-siR255 is absent from heading Chinese cabbage (Brassica rapa ssp. pekinensis). Our previous attempt to overexpress the endogenous BrpHTT2 gene of heading Chinese cabbage (B. rapa ssp. pekinensis) failed because of cosuppression. In theory, heading Chinese cabbage can overexpress Arabidopsis HTT2 to improve thermotolerance in the absence of ta-siR255-mediated gene silencing and the weak potential of coexpression.Results:To test the potential application of HTT2 in improving crop thermotolerance, we transferred p35S::HTT2 to heading Chinese cabbage. We tested the leaf electrical conductivity, hypocotyl elongation, and survival percentage of p35S::HTT2 plants subjected to high-temperature (38 °C) and heat-shock (46 °C) treatment. The leaf electrical conductivity of p35S::HTT2 seedlings under high temperature decreased but did negligibly change under heat shock. The hypocotyl length of p35S::HTT2 seedlings increased under high temperature and heat shock. The survival rate of p35S::HTT2 seedlings increased under heat shock. BrpHsfs, a subset of heat-shock factor genes, were upregulated in p35S::HTT2 plants under high-temperature and heat shock conditions. In the field, transgenic plants with HTT2 appeared greener and formed leafy heads earlier than wild-type plants.Conclusions:Exogenous HTT2 increased the survival rates of heat-shocked heading Chinese cabbage by promoting thermotolerance through decreasing electrical conductivity and extending hypocotyl length. Our work provides a new approach to the genetic manipulation of thermotolerance in crops through the introduction of exogenous thermotolerance genes.
登录
查看更多内容
DOI：
10.1007/s004380050731
发表时间：
1998-05-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Prändl, R;Hinderhofer, K;Schöffl, F
通讯作者：
Schöffl, F
影响因子：
9.2
作者：
Park, W;Li, JJ;Chen, XM
通讯作者：
Chen, XM
影响因子：
11.6
作者：
Li, Shuxia;Liu, Jinxin;He, Yuke
通讯作者：
He, Yuke
影响因子：
1.6
作者：
Kume, Kohei;Tsutsumi, Ken-ichi;Saitoh, Yasushi
通讯作者：
Saitoh, Yasushi
影响因子：
11.6
作者：
Sunkar, R;Zhu, JK
通讯作者：
Zhu, JK