Production of viable seeds from the seedling lethal mutant ppi2-2 lacking the atToc159 chloroplast protein import receptor using plastic containers, and characterization of the homozygous mutant progeny.
Production of viable seeds from the seedling lethal mutant ppi2-2 lacking the atToc159 chloroplast protein import receptor using plastic containers, and characterization of the homozygous mutant progeny.
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DOI：
10.3389/fpls.2014.00243
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发表时间：
2014
影响因子：
5.6
通讯作者：
Inaba T
中科院分区：
文献类型：
--
作者：
Tada A;Adachi F;Kakizaki T;Inaba T
叶绿体的生物发生对植物的生长发育至关重要。许多缺乏叶绿体蛋白的纯合突变体表现出白化病表型。一般来说，在土壤上生长白化病拟南芥植物直到它们结种子是具有挑战性的。纯合的白化病突变体通常作为杂合亲本的后代获得。在这里，我们描述了一种方法，从幼苗致死拟南芥突变体ppi 2 -2，它缺乏在叶绿体外被膜atToc 159蛋白输入受体恢复种子。使用塑料容器，我们能够种植纯合ppi 2 -2植物，直到这些植物结籽。虽然收获的种子的发芽率相对较低，但仍足以允许我们进一步分析ppi 2 -2后代。使用ppi 2 -2纯合种子，我们能够分析质体蛋白质输入在光调控诱导核基因中的作用。我们建议将这种方法应用于其他幼苗致死拟南芥突变体，以获得纯合种子，帮助我们进一步研究质体蛋白在植物生长发育中的作用。
Biogenesis of chloroplasts is essential for plant growth and development. A number of homozygous mutants lacking a chloroplast protein exhibit an albino phenotype. In general, it is challenging to grow albino Arabidopsis plants on soil until they set seeds. Homozygous albino mutants are usually obtained as progenies of heterozygous parents. Here, we describe a method of recovering seeds from the seedling lethal Arabidopsis mutant ppi2-2, which lacks the atToc159 protein import receptor at the outer envelope membrane of chloroplast. Using plastic containers, we were able to grow homozygous ppi2-2 plants until these set seed. Although the germination rate of the harvested seeds was relatively low, it was still sufficient to allow us to further analyze the ppi2-2 progeny. Using ppi2-2 homozygous seeds, we were able to analyze the role of plastid protein import in the light-regulated induction of nuclear genes. We propose that this method be applied to other seedling lethal Arabidopsis mutants to obtain homozygous seeds, helping us further investigate the roles of plastid proteins in plant growth and development.
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影响因子：
7.2
作者：
Motohashi, R;Ito, T;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
7.2
作者：
Constan, D;Patel, R;Jarvis, P
通讯作者：
Jarvis, P
影响因子：
11.6
作者：
Kubis, S;Patel, R;Jarvis, P
通讯作者：
Jarvis, P
质体蛋白易位通道的完全成熟需要I型信号肽酶。
影响因子：
7.8
作者：
Inoue, Kentaro;Baldwin, Amy J;Shipman, Rebecca L;Matsui, Kyoko;Theg, Steven M;Ohme-Takagi, Masaru
通讯作者：
Ohme-Takagi, Masaru
影响因子：
56.9
作者：
KESSLER, F;BLOBEL, G;SCHNELL, DJ
通讯作者：
SCHNELL, DJ