Wound healing monitoring using near infrared fluorescent fibrinogen.
Wound healing monitoring using near infrared fluorescent fibrinogen.
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DOI：
10.1364/boe.1.000285
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发表时间：
2010-07-27
影响因子：
3.4
通讯作者：
Faris GW
中科院分区：
文献类型：
--
作者：
Pan CP;Shi Y;Amin K;Greenberg CS;Haroon Z;Faris GW
我们展示了一种用近红外荧光纤维蛋白原对伤口愈合过程进行成像的方法。在大鼠穿孔活检模型上进行伤口愈合研究。纤维蛋白原与近红外荧光染料结合并注射到尾静脉中。纤维蛋白原是追踪伤口愈合的有用蛋白质，因为它参与纤维蛋白凝块的形成以及通过转谷氨酰胺酶的交联活性形成新的临时基质。观察到伤口特异的强荧光并持续数天，表明纤维蛋白原转化为交联纤维蛋白。在伤口形成的同时施用造影剂导致纤维蛋白凝块的初步标记，表明伤口处于愈合的早期阶段。第二天的给药显示伤口周围有标记，表明新的临时基质的形成位置。该方法可能被证明可用作伤口愈合过程基础研究、药物开发或慢性伤口临床评估的诊断方法。
We demonstrate a method for imaging the wound healing process with near infrared fluorescent fibrinogen. Wound healing studies were performed on a rat punch biopsy model. Fibrinogen was conjugated with a near infrared fluorescent dye and injected into the tail vein. Fibrinogen is a useful protein for tracking wound healing because it is involved in fibrin clot formation and formation of new provisional matrix through transglutaminase’s crosslinking activity. Strong fluorescence specific to the wound was observed and persisted for several days, indicating that the fibrinogen is converted to crosslinked fibrin. Administration of contrast agent simultaneously with wound creation led to primary labeling of the fibrin clot, indicating that the wound was in its early phase of healing. Administration on the following day showed labeling on the wound periphery, indicating location of formation of a new provisional matrix. This method may prove to be useful as a diagnostic for basic studies of the wound healing process, in drug development, or in clinical assessment of chronic wounds.
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影响因子：
3.5
作者：
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