Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
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DOI：
10.1371/journal.pone.0062248
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sundsfjord A
中科院分区：
文献类型：
--
作者：
Song X;Sun J;Mikalsen T;Roberts AP;Sundsfjord A
本研究的目的是确定和表征质粒在一个国家收集的口腔粪肠球菌（n = 106）边缘牙周炎患者分离。 质粒复制子分型进行了多重PCR和测序的18个重复家族和1个独特的序列的特异性引物。通过S1-PFGE进行额外的质粒分析以进行比较。在93株大肠杆菌中共检测到7个复制子家族的120个质粒复制子。粪肠球菌菌株，例如rep9（原型pCF 10）、rep6（原型pS86）、rep2（原型pRE 25/pEF 1）和rep8（原型pAM 373）。Rep9是81株（76.4%）菌株中最主要的rep-family。这些菌株中有40株是四环素耐药的，3株是红霉素耐药的。Rep6是第二大优势家族，在22株（20.8%）菌株中检测到。Rep2在8株（7.5%）菌株中检出。所有rep2阳性菌株均对四环素和/或红霉素耐药，其中6株含有Tn916/Tn1545基因。阳性E.粪肠球菌表现出分歧的多位点序列类型（ST）。rep9与ST21之间存在显著相关性，而ST40中出现多个rep-family。在95株E. S1-PFGE结果显示，59株携带1个质粒，27株携带2个质粒，5株携带3个质粒，3株携带4个质粒，1株携带5个质粒。质粒大小在5 - 150 kbp之间变化。PCR rep-typing和S1-PFGE鉴定的质粒数量之间存在显著相关性。结果表明，绝大多数E.边缘性牙周炎的粪便可能是多种移动的遗传因子和相关的抗菌素耐药性决定因素的储存库。
The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. Additional plasmid analysis by S1-PFGE was performed for comparison. Totally 120 plasmid replicon amplicons of seven rep-families were identified in 93 E. faecalis strains, e.g. rep9 (prototype pCF10), rep6 (prototype pS86), rep2 (prototype pRE25/pEF1), and rep8 (prototype pAM373). Rep9 was the most predominant rep-family being detected in 81 (76.4%) strains. Forty of these strains were tetracycline resistant and three were erythromycin resistant. Rep6 was the second predominant rep-family being detected in 22 (20.8%) strains. Rep2 was detected in eight (7.5%) strains. All rep2-positive strains were resistant to tetracycline and/or erythromycin and six of them contained Tn916/Tn1545 genes. The rep-positive E. faecalis exhibited divergence in multilocus sequence types (STs). There was a significant correlation between rep9 and ST21, while multiple rep-families appeared in ST40. Totally 145 plasmid bands were identified in 95 E. faecalis strains by S1-PFGE, 59 strains carrying one plasmid, 27 carrying two, five carrying three, three carrying four, and one strain carrying five plasmids. Plasmid sizes varied between 5–150 kbp. There was a significant correlation between the number of plasmids identified by PCR rep-typing and by S1-PFGE. The results indicate that the majority of E. faecalis of marginal periodontitis are likely to be a reservoir for diverse mobile genetic elements and associated antimicrobial resistance determinants.
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影响因子：
3.3
作者：
Ng, LK;Martin, I;Mulvey, M
通讯作者：
Mulvey, M
影响因子：
--
作者：
Dahlén, G;Samuelsson, W;Reit, C
通讯作者：
Reit, C
影响因子：
9.4
作者：
TENOVER, FC;ARBEIT, RD;SWAMINATHAN, B
通讯作者：
SWAMINATHAN, B
影响因子：
9.4
作者：
Carvalho, MDS;Steigerwalt, AG;Facklam, RR
通讯作者：
Facklam, RR
影响因子：
--
作者：
Rosvoll, Torill C. S.;Lindstad, Belinda L.;Pedersen, Torunn
通讯作者：
Pedersen, Torunn