Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population.
Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population.
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DOI：
10.1186/1471-2180-9-175
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发表时间：
2009-08-23
期刊：
影响因子：
4.2
通讯作者：
Yamaoka Y
中科院分区：
文献类型：
--
作者：
Uchida T;Nguyen LT;Takayama A;Okimoto T;Kodama M;Murakami K;Matsuhisa T;Trinh TD;Ta L;Ho DQ;Hoang HH;Kishida T;Fujioka T;Moriyama M;Yamaoka Y
亚洲各国胃癌的发病率有所不同，有人认为与幽门螺杆菌相关的毒力因素是部分原因。本研究的目的是调查越南受试者幽门螺杆菌分离物中被视为毒力或分子流行病学标记的几种遗传因素。通过PCR和测序测定了来自越南的103株幽门螺杆菌（54株来自河内，49株来自胡志明市）的cagA、vacA和cag右端连接基因型。鉴定出位于cagA Glu-Pro-Ile-Tyr-Ala (EPIYA)重复区上游区域的三种类型的缺失：39-bp缺失型、18-bp缺失型和无缺失型。大多数研究菌株（77%；80/103）都存在 18 bp 缺失，无论该国的地理位置或临床结果如何。所有39 bp和18 bp缺失型菌株均具有东亚型cagA重复区域。 II 型 cag 右端连接基因型占主导地位 (84%)。 vacA m1 基因型在胃癌发病率较高的河内分离菌株中比胡志明菌株中更为常见。 cagA 基因的前 EPIYA 区域分型可以提供幽门螺杆菌基因组多样性的新遗传标记。我们的数据支持 vacA m1 与胃癌发生密切相关的假设。
The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with Helicobacter pylori are partly responsible. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic markers in H. pylori isolates from Vietnamese subjects. The cagA, vacA and cag right-end junction genotypes of 103 H. pylori strains from Vietnam (54 from Hanoi and 49 from Ho Chi Minh) were determined by PCR and sequencing. Three types of deletion in the region located upstream of the cagA Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region were identified: the 39-bp deletion type, the 18-bp deletion type, and the no-deletion type. The majority of strains studied (77%; 80/103) had the 18-bp deletion irrespective of geographical location in the country or clinical outcome. All of the 39-bp and 18-bp deletion-type strains possessed the East Asian type cagA repeat region. The type II cag right-end junction genotype was predominant (84%). The vacA m1 genotype was significantly more common in strains isolated in Hanoi, where the incidence of gastric cancer is higher, than in strains from Ho Chi Minh. Pre-EPIYA-region typing of the cagA gene could provide a new genetic marker of H. pylori genomic diversity. Our data support the hypothesis that vacA m1 is closely associated with gastric carcinogenesis.
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影响因子：
3.1
作者：
Occhialini, A;Marais, A;Mégraud, F
通讯作者：
Mégraud, F
DOI：
10.1093/jnci/87.23.1777
发表时间：
1995-12-06
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
KUIPERS, EJ;PEREZPEREZ, GI;BLASER, MJ
通讯作者：
BLASER, MJ
影响因子：
9.4
作者：
Heep, M;Scheibl, K;Lehn, N
通讯作者：
Lehn, N
影响因子：
29.4
作者：
van Doorn, LJ;Figueiredo, C;Queiroz, DMD
通讯作者：
Queiroz, DMD
影响因子：
3.5
作者：
Yamaoka, Y;Orito, E;Graham, DY
通讯作者：
Graham, DY